vestichИзвестия Национальной академии наук Беларуси. Серия химических наукProceedings of the National Academy of Sciences of Belarus, Chemical Series1561-83312524-2342The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"vestich-197Research ArticleБИООРГАНИЧЕСКАЯ ХИМИЯBIOORGANIC CHEMISTRYМОЛЕКУЛЯРНОЕ КЛОНИРОВАНИЕ, ГЕТЕРОЛОГИЧЕСКАЯ ЭКСПРЕССИЯ И ПОЛУЧЕНИЕ РЕКОМБИНАНТНОГО СОМАТОТРОПИНА GALLUS GALLUSMOLECULAR CLONING, HETEROLOGICAL EXPRESSION AND PRODUCTION OF THE RECOMBINANT G. GALLUS GROWTHГорькаваяА. М.GorkavayaA. M.annagorkavaya@gmail.comСергеевГ. В.SergeevG. V.gvserg@iboch.bas-net.byГилепА. А.GilepA. A.agilep@iboch.bas-net.byИнститут биоорганической химии НАН БеларусиInstitute of Bioorganic Chemistry of the National Academy of Sciences of Belarus201607082016027682Copyright © Горькавая А.М., Сергеев Г.В., Гилеп А.А., 20162016Горькавая А.М., Сергеев Г.В., Гилеп А.А.Gorkavaya A.M., Sergeev G.V., Gilep A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestichem.belnauka.by/jour/article/view/197

Соматотропины относятся к группе полипептидных гормонов, запускающих различные каскады биохимиче­ских процессов. Основная функция соматотропина – влияние на анаболические и катаболические процессы орга­низма, а также иммуномодулирующая функция. В представленной работе проведено молекулярное клонирование, гетерологическая экспрессия и очистка рекомбинантного соматотропина Gallus gallus. Созданы две технологиче­ские схемы получения соматотропина: бактериальная экспрессия с последующей солюбилизацией телец включения и периплазматическая экспрессия соматотропина в бактериальных клетках. Выход целевого гормона составил 2,43 и 15 мг белка на литр культуральной среды соответственно. В работе показано, что наиболее оптимальной является стратегия периплазматической экспрессии, при которой нет необходимости в сложных процедурах солюбилизации и рефолдинга и получаемый белковый продукт характеризуется более высоким выходом и степенью очистки.

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The authors declare that there are no conflicts of interest present.