vestichИзвестия Национальной академии наук Беларуси. Серия химических наукProceedings of the National Academy of Sciences of Belarus, Chemical Series1561-83312524-2342The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8331-2019-55-3-299-308vestich-407Research ArticleСтатьиГибридные биоматериалы на основе гидроксиапатита и компонентов кровиHibrid biomaterials based on hydroxyapatite and blood componentsКрутькоВ. К.Krut’koV. K.

Крутько Валентина Константиновна – канд. хим. наук, доцент, вед. науч. сотрудник

Valentina K. Krut’ko – Ph. D. (Chemistry), Associate professor, Leading researcher

9/1, Surganov Str., 220072, Minsk

tsuber@igic.bas-net.byВласовР. А.VlasovR. A.

Власов Роман Алексеевич – ЛОР-врач

Roman A. Vlasov – ENT specialist

rvalekseevich@mail.ruМусскаяО. Н.MusskayaO. N.

Мусская Ольга Николаевна – канд. хим. наук, доцент, ст. науч. сотрудник

Olga N. Musskaya – Ph. D. (Chemistry), Associate professor, Senior researcher

musskaja@igic.bas-net.byГлазовИ. Е.GlazovI. E.

Глазов Илья Евгеньевич – мл. науч. сотрудник

Ilya E. Glazov – Junior researcher

che.glazov@mail.ruКулакА. И.KulakA. I.

Кулак Анатолий Иосифович – член-корреспондент, д-р хим. наук, профессор, директор

Anatoly I. Kulak – Corresponding Member of the National Academy of Sciences of Belarus, D. Sc. (Chemistry), Professor, Director

9/1, Surganov Str., 220072, Minsk

kulak@igic.bas-net.byИнститут общей и неорганической химии Национальной академии наук БеларусиInstitute of General and Inorganic Chemistry of National Academy of Sciences of BelarusРеспубликанский госпиталь Департамента финансов и тыла Министерства внутренних дел Республики БеларусьRepublican Hospital of Department of Finance and Logistics of the Ministry of Internal Affairs of Republic of Belarus201912092019553299308Copyright © Крутько В.К., Власов Р.А., Мусская О.Н., Глазов И.Е., Кулак А.И., 20192019Крутько В.К., Власов Р.А., Мусская О.Н., Глазов И.Е., Кулак А.И.Krut’ko V.K., Vlasov R.A., Musskaya O.N., Glazov I.E., Kulak A.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestichem.belnauka.by/jour/article/view/407

Разработаны гибридные биоматериалы на основе аморфизированного гидроксиапатита и компонентов крови (фибрин, цитратная плазма) химическим осаждением гидроксиапатита в биополимерной матрице (pH 11; соотношении Са/Р 1,67) и путем смешивания 6–14 мас.% геля гидроксиапатита (pH 7,0–7,2) с биполимерами. Химически осажденный в биополимерных матрицах гидроксиапатит является однофазным либо с примесью трикальцийфосфата до 30 %, преимущественно α-модификации в фибриновой матрице и β-модификации в цитратной плазме. Взаимодействие геля гидроксиапатита с фибрином приводит к значительной аморфизации гидроксиапатита и повышению его биорезорбируемости. Выдерживание композитов с гидроксиапатитом, полученным химическим осаждением, в модельном растворе Simulated Body Fluid в течение 75 сут приводит к их частичной резорбции и одновременному нарастанию биомиметического апатита, с бóльшим его приростом по массе на композитах с фибрином. Гибридные биоматериалы на основе фибрина, полученного из крови пациента, и геля гидроксиапатита показали положительный результат при имплантации, позволяя сформировать адекватную конфигурацию дефекта, расширяя возможности ЛОР-хирургии.

Hybrid biomaterials based on amorphous hydroxyapatite and blood components (fibrin, citrate plasma) were developed by chemical precipitation of hydroxyapatite in a biopolymer matrix (pH 11; Ca/P ratio 1.67) and by mixing 6–14 wt.% of hydroxyapatite gel (pH 7.0–7.2) with bipolymers. Chemically precipitated hydroxyapatite in biopolymer matrices is single phase or contains ticalcium phosphate impurity up to 30 %, mainly α-modification in fibrin matrix and β-modification in citrate plasma. The interaction of hydroxyapatite gel into the fibrin leads to significant amorphization of hydroxyapatite and an increase in its bioresorbability. Holding the composites with hydroxyapatite obtained by chemical precipitation in the Simulated Body Fluid model solution for 75 days leads to their partial resorption and simultaneous increase of biomimetic apatite, with its greater weight gain on composites with a fibrin. Hybrid biomaterials based on a fibrin obtained from the patient’s blood and hydroxyapatite gel showed positive result during implantation, allowing to form an adequate configuration of the defect, expanding the possibilities of ENT surgery.

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The authors declare that there are no conflicts of interest present.