vestichИзвестия Национальной академии наук Беларуси. Серия химических наукProceedings of the National Academy of Sciences of Belarus, Chemical Series1561-83312524-2342The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8331-2020-56-4-391-398vestich-610Research ArticleФИЗИЧЕСКАЯ ХИМИЯPHYSICAL CHEMISTRYМикросферы из сшитого глутаральдегидом поливинилового спирта для иммуно-магнитной сепарации клетокMicrospheres made of polyvinyl alcohol crosslinked with glutaraldehyde for immunomagnetic cell separationЛазневК. В.LaznevK. V.

Лазнев Константин Владимирович - научный сотрудник.

Ул. Ф. Скорины, 36, 220141, Минск

Konstantin V. Laznev - Researcher.

36, F. Skaryna Str., 220141, Minsk

kvlaznev@mail.ruАгабековВ. Е.AgabekovV. E.

Агабеков Владимир Енокович - академик, доктор химических наук, профессор, директор.

Ул. Ф. Скорины, 36, 220141, Минск

Vladimir Ye. Agabekov - Academician, D. Sc. (Chemistry), Professor, Director.

ichnm@ichnm.basnet.byИнститут химии новых материалов, Национальная академия наук БеларусиInstitute of Chemistry of New Materials of the National Academy of Science of Belarus202004122020564391398Copyright © Лазнев К.В., Агабеков В.Е., 20202020Лазнев К.В., Агабеков В.Е.Laznev K.V., Agabekov V.E.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestichem.belnauka.by/jour/article/view/610

Установлено, что эмульгируемость водных растворов NaCl и CaCl2 в изооктане с добавками Span 85 и Tween 85 зависит от концентрации солей и от природы катиона, при этом Ca2+ эффективнее стабилизирует эмульсию, чем Na+. При смешивании растворов 275 г/л глутаральдегида и 1М CaCl2 в воде и 2,8 %m Span 85 и 1,5 %m Tween 85 в изооктане с последующим ультразвуковым диспергированием смеси получена эмульсия с мутностью <20 ЕМФ, которая была применена в качестве сшивающего агента для формирования микросфер из взвешенных в изооктане микрокапель магнитных жидкостей, содержащих поливиниловый спирт и наночастицы магнетита. Синтезированные магнитные микросферы модифицированы авидином и использованы для иммуномагнитной сепарации клеток.

Emulsification of water solutions of NaCl and CaCl2 in isooctane with Span 85 and Tween 85 additives was found to depend on the salt content and the cation nature, Ca2+ being more effective stabilizer than Na+. When the solutions of 275 g/l glutaraldehyde with 1М CaCl2 in water and 2.8 %m Span 85 with 1.5 %m Tween 85 in isooctane were mixed and sonicated, the transparent emulsion (turbidity <20 FNU) was acquired. It was used as а crosslinking agent to synthesize microspheres from magnetic fluids containing polyvinyl alcohol and magnetite nanoparticles dispersed as microdroplets in isooctane. The microspheres were modified with avidin and used for immunomagnetic separation of cells.

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The authors declare that there are no conflicts of interest present.