vestichИзвестия Национальной академии наук Беларуси. Серия химических наукProceedings of the National Academy of Sciences of Belarus, Chemical Series1561-83312524-2342The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8331-2021-57-2-206-217vestich-650Research ArticleБИООРГАНИЧЕСКАЯ ХИМИЯBIOORGANIC CHEMISTRYМолекулярно-структурная гомология протеолитических ферментов в изучении механизма протеолиза и его регуляцииMolecular-structural homology of proteolytic enzymеs in the studying of proteolysis mechanism and its regulationЧиркинА. А.ChirkinA. A.

Чиркин Александр Александрович – д-р биол. наук, профессор.

Московский пр. 33, 210038, Витебск 

Alexander A. Chirkin – D. Sc. (Biology), Professor

33, Moskovsky Ave, 210038, Vitebsk 

chir@tut.byБалаева-ТихомироваО. М.Balaeva-TikhomirovaO. M.

Балаева-Тихомирова Ольга Михайловна – канд. биол. наук, доцент, зав. кафедрой

Московский пр. 33, 210038, Витебск

Olga M. Balaeva-Tikhomirova – Ph. D. (Biology), Associate Professor, Head of the Department

33, Moskovsky Ave, 210038, Vitebsk 

olgabal.tih@gmail.comДолматоваВ. В.DolmatovaV. V.

Долматова Виктория Викторовна – аспирант

Московский пр. 33, 210038, Витебск

Victoria V. Dolmatova – Postgraduate Student

33, Moskovsky Ave, 210038, Vitebsk 

vika.dolmatova19@yandex.byСеменовИ. О.SemenovI. O.

Семенов Игорь Олегович – магистрант

Московский пр. 33, 210038, Витебск

Igor O. Semenov – Master’s Student

33, Moskovsky Ave, 210038, Vitebsk 

igor.semionov.96@br.ruВитебский государственный университет имени П. М. МашероваVitebsk State University named after P. M. Masherov202103062021572206217Copyright © Чиркин А.А., Балаева-Тихомирова О.М., Долматова В.В., Семенов И.О., 20212021Чиркин А.А., Балаева-Тихомирова О.М., Долматова В.В., Семенов И.О.Chirkin A.A., Balaeva-Tikhomirova O.M., Dolmatova V.V., Semenov I.O.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestichem.belnauka.by/jour/article/view/650

В современной медицине животные рассматриваются как модельные организмы для различных патологий при доклинических испытаниях и источники биологического материала для заместительной терапии. Целью исследования явился сравнительный анализ степени гомологии протеолитических ферментов у человека и легочных пресноводных моллюсков. В ходе исследования установлено, что гомология ферментов по нуклеотидным последовательностям у человека и легочных пресноводных моллюсков при анализе нерегулируемого протеолиза составляет 66–68 %; регулируемого протеолиза – 69–76 %; убиквитин-подобных модификаторов – 78–83 %; внеклеточных ферментов – 67–76 % и внутриклеточных ферментов – 65–72 %. Эволюционный консерватизм протеолитических ферментов позволяет использовать этих животных в качестве дешевых и удобных в содержании тест-организмов и обосновывает целесообразность формирования аквакультуры моллюсков, для получения из их тканей белковых ферментативных препаратов протеолитического действия в рамках задач биофармацевтики, косметики и пищевой промышленности.

The actual problem of experimental medicine is the substantiation of new model organisms that meet modern requirements of bioethics, cost and conditions of detention. The aim of this work was a comparative analysis of the homology degree of proteolytic enzymes in humans and pulmonary freshwater mollusks. The homology of enzymes in nucleotide sequences in humans and pulmonary freshwater mollusks in the analysis of unregulated proteolysis is 66–68 %; regulated proteolysis – 69–76 %; ubiquitin-like modifiers – 78–83 %; extracellular enzymes – 67–76 %; and intracellular enzymes – 65–72 %. The evolutionary conservatism of proteolytic enzymes and the presence of an open blood circulation, which allows the substances under study to be delivered from the hemolymph directly to target cells, make it possible to use these animals as cheap and convenient test organisms. The practical importance of a sufficiently high homology degree of proteolytic enzymes in humans and pulmonary freshwater mollusks justifies the expediency of forming mollusk aquaculture to obtain proteolytic enzyme protein preparations from their tissues within the framework of the tasks of biopharmaceuticals, cosmetics and the food industry.

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The authors declare that there are no conflicts of interest present.