vestichИзвестия Национальной академии наук Беларуси. Серия химических наукProceedings of the National Academy of Sciences of Belarus, Chemical Series1561-83312524-2342The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8331-2021-57-3-300-309vestich-666Research ArticleБИООРГАНИЧЕСКАЯ ХИМИЯBIOORGANIC CHEMISTRYРазработка мультиплексной системы для определения 11 генетических маркеров предрасположенности к развитию ожиренияDevelopment of a multiplex system for determining 11 genetic markers of predisposition to obesityСергеевГ. В.SergeevG. V.

Геннадий Валерьевич Сергеев – канд. хим. наук, зав. лаб.

ул. Акад. В. Ф. Купревича, 5/2, 220141, Минск

Gennady V. Sergeev – Ph. D. (Chemistry), Head of the Laboratory

5/2, Acad. Kuprevich Str., Minsk 

gvserg@iboch.byГайдукевичИ. В.HaidukevichI. V.

Ирина Витальевна Гайдукевич – канд. хим. наук, ст. науч. сотрудник

ул. Акад. В. Ф. Купревича, 5/2, 220141, Минск

Irina V. Haidukevich – Ph. D. (Chemistry), Senior Researcher

5/2, Acad. Kuprevich Str., Minsk 

ribano4ka@gmail.comГорькаваяА. М.GorkavayaA. M.

Анна Михайловна Горькавая – науч. сотрудник

ул. Акад.  В. Ф. Купревича, 5/2, 220141, Минск

Anna M. Gorkavaya – Researcher

5/2, Acad. Kuprevich Str., Minsk  

annagorkavaya@gmail.comАмельяновичМ. Д.AmeliyanovichM. D.

Максим Дмитриевич Амельянович – науч. сотрудник

ул. Академическая, 27, 220072, Минск

Maxim D. Ameliyanovich – Researcher

27, Akademicheskaya str., Minsk 

M.Ameliyanovich@igc.byМоссэИ. Б.MosseI. B.

Ирма Борисовна Моссэ – д-р биол. наук, профессор, гл. науч. сотрудник

ул. Академическая, 27, 220072, Минск

Irma B. Mosse – D. S. (Biology), Professor, Principal Researcher

27, Akademicheskaya str., Minsk 

I.Mosse@igc.byУсановС. А.UsanovS. A.

Сергей Александрович Усанов – член-корреспондент, д-р хим. наук, профессор, гл. науч. сотрудник

ул. Акад. В. Ф. Купревича, 5/2, 220141, Минск

Sergei A. Usanov – Corresponding Member of the National Academy of Sciences of Belarus, D. S. (Chemistry), Professor, Principal Researcher

5/2, Acad. Kuprevich Str., Minsk 

usanov@iboch.byИнститут биоорганической химии Национальной академии наук БеларусиInstitute of Bioorganic Chemistry of the National Academy of Sciences of BelarusИнститут генетики и цитологии Национальной академии наук БеларусиInstitute of Genetics and Cytology of the National Academy of Sciences of Belarus202103092021573300309Copyright © Сергеев Г.В., Гайдукевич И.В., Горькавая А.М., Амельянович М.Д., Моссэ И.Б., Усанов С.А., 20212021Сергеев Г.В., Гайдукевич И.В., Горькавая А.М., Амельянович М.Д., Моссэ И.Б., Усанов С.А.Sergeev G.V., Haidukevich I.V., Gorkavaya A.M., Ameliyanovich M.D., Mosse I.B., Usanov S.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestichem.belnauka.by/jour/article/view/666

С помощью метода минисеквенирования (SNaPshot анализ) разработана система для определения 11 генетических маркеров, связанных с риском развития ожирения: rs10852521, rs11075990, rs1121980, rs1421085, rs1477196, rs17817449, rs3751812, rs7206790, rs8047395, rs9940128 (ген FTO) и rs1137101 (ген LEPR). Условия проведения реакций амплификации и минисеквенирования, а также составы реакционных смесей оптимизированы так, чтобы проводить анализ по всем 11 маркерам одновременно. Полученная система апробирована и показала высокую степень воспроизводимости и чувствительности, необходимой для определения данных полиморфных вариантов.

A system has been developed to identify 11 genetic markers associated with the risk of obesity: rs10852521, rs11075990, rs1121980, rs1421085, rs1477196, rs17817449, rs3751812, rs7206790, rs8047395, rs9940128 (FTO gene) and rs1137101 (LEPR gene) by minisequencing (SNaPshot analysis). The conditions for carrying out the amplification and minisequencing reactions, as well as the compositions of the reaction mixtures, were optimized so that the analysis was carried out for all 11 markers simultaneously. The resulting system was tested and showed a high degree of reproducibility and sensitivity required for the detection of these polymorphisms.

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The authors declare that there are no conflicts of interest present.