vestichИзвестия Национальной академии наук Беларуси. Серия химических наукProceedings of the National Academy of Sciences of Belarus, Chemical Series1561-83312524-2342The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8331-2022-58-2-135-148vestich-716Research ArticleФИЗИЧЕСКАЯ ХИМИЯPHYSICAL CHEMISTRYФормирование микроструктурированных пленок поли-3-оксибутирата с регулируемой топографией поверхностиFabrication of microstructured poly(3-hydroxybutyrate) films with controlled surface topographyhttps://orcid.org/0000-0001-6505-3929КуликовскаяВ. И.KulikouskayaV. I.

Куликовская Виктория Игоревна - кандидат химических наук, доцент, заместитель директора.

ул. Ф. Скорины, 36, 220141, Минск.

Viktoryia I. Kulikouskaya - Ph. D. (Chemistry), Associate Professor, Deputy Director, Institute of Chemistry of New Materials of the National Academy of Sciences of Belarus.

36, F. Skorina str., 220141, Minsk.

kulikouskaya@gmail.comНиколайчукВ. В.NikalaichukV. V.

Николайчук Виктория Викторовна - магистрант, младший научный сотрудник.

ул. Ф. Скорины, 36, 220141, Минск.

Viktoryia V. Nikalaichuk - Master student, Junior Researcher, Institute of Chemistry of New Materials of the National Academy of Sciences of Belarus.

36, F. Skorina str., 220141, Minsk.

vica10bcn@gmail.comhttps://orcid.org/0000-0001-5894-9524БонарцевА. П.BonartsevA. P.

Бонарцев Антон Павлович - кандидат биологических наук, доцент.

Ленинские горы, д. 1, стр. 12, 199234, Москва.

Anton P. Bonartsev - Ph. D. (Biology), Associate Professor, Lomonosov Moscow State University.

1, Bld. 12, Leninskie gory, 119071, Moscow.

ant_bonar@mail.ruЧишанковИ. Г.ChyshankouI. G.

Чишанков Игнат Геннадьевич - младший научный сотрудник.

ул. Ф. Скорины, 36, 220141, Минск.

Ihnat G. Chyshankou - Junior Researcher, Institute of Chemistry of New Materials of the National Academy of Sciences of Belarus.

36, F. Skorina str., 220141, Minsk.

chishankov_ignat@mail.ruhttps://orcid.org/0000-0002-2560-624XАкулинаЕ. А.AkoulinaE. A.

Акулина Елизавета Александровна - младший научный сотрудник.

Ленинские горы, д. 1, стр. 12, 199234, Москва.

Elizaveta A. Akoulina - Junior Researcher, Lomonosov Moscow State University.

1, Bld. 12, Leninskie gory, 119071, Moscow.

akoulinaliza@gmail.comhttps://orcid.org/0000-0002-5208-8775ДемьяноваИ. В.DemianovaI. V.

Демьянова Ирина Валерьевна - младший научный сотрудник.

Ленинские горы, д. 1, стр. 12, 199234, Москва.

Irina V. Demianova - Junior Researcher, Lomonosov Moscow State University.

1, Bld. 12, Leninskie gory, 119071, Moscow.

irinydem@yandex.comБонарцеваГ. А.BonartsevaG. A.

Бонарцева Гарина Александровна - кандидат биологических наук, ведущий научный сотрудник.

Ленинский проспект, д. 33, стр. 2, 119071, Москва.

Garina A. Bonartseva - Ph. D. (Biology), Senior Researcher, Research Center of Biotechnology of the Russian Academy of Sciences.

33, Bld. 2, Leninsky Ave, 119071, Moscow.

bonar@inbi.ras.ruhttps://orcid.org/0000-0002-3121-0014ГилевскаяК. С.HileuskayaК. S.

Гилевская Ксения Сергеевна - кандидат химических наук, доцент, ведущий научный сотрудник.

ул. Ф. Скорины, 36, 220141, Минск.

Kseniya S. Hileuskaya - Ph. D. (Chemistry), Associate Professor, Leading Researcher, Institute of Chemistry of New Materials of the National Academy of Sciences of Belarus.

36, F. Skorina str., 220141, Minsk.

k_hilevskay@mail.ruhttps://orcid.org/0000-0002-0253-6461ВоиноваВ. В.VoinovaV. V.

Воинова Вера Владимировна - кандидат биологических наук, старший научный сотрудник.

Ленинские горы, д. 1, стр. 12, 199234, Москва.

Vera V. Voinova - Ph. D. (Biology), Senior Researcher, Lomonosov Moscow State University.

1, Bld. 12, Leninskie gory, 119071, Moscow.

veravoinova@mail.ruИнститут химии новых материалов Национальной академии наук БеларусиInstitute of Chemistry of New Materials of the National Academy of Sciences of BelarusМосковский государственный университет имени М.В. ЛомоносоваLomonosov Moscow State UniversityФИЦ Фундаментальные основы биотехнологии РАНResearch Center of Biotechnology of the Russian Academy of Sciences202208062022582135148Copyright © Куликовская В.И., Николайчук В.В., Бонарцев А.П., Чишанков И.Г., Акулина Е.А., Демьянова И.В., Бонарцева Г.А., Гилевская К.С., Воинова В.В., 20222022Куликовская В.И., Николайчук В.В., Бонарцев А.П., Чишанков И.Г., Акулина Е.А., Демьянова И.В., Бонарцева Г.А., Гилевская К.С., Воинова В.В.Kulikouskaya V.I., Nikalaichuk V.V., Bonartsev A.P., Chyshankou I.G., Akoulina E.A., Demianova I.V., Bonartseva G.A., Hileuskaya К.S., Voinova V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestichem.belnauka.by/jour/article/view/716

Исследована возможность формирования микроструктурированных пленок поли-3-оксибутирата методом «самоорганизации» микрокапель воды с помощью искусственных шаблонов и обратных эмульсий полимера. Установлено, что методом «самоорганизации» можно сформировать упорядоченные микроструктуры поли-3-оксибутирата с гексагональным расположением ячеек регулируемого диаметра от 1 до 4 мкм. Показано, что путем применения обратных эмульсий поли-3-оксибутирата можно получить пористые пленки с заданным размером пор от 0,4 до 3 мкм, при этом структуру пленок и размер пор в них можно регулировать путем изменения концентрации полимера в дисперсионной среде и объемного соотношения фаз. С помощью метода центрифугирования и применения искусственных шаблонов можно создавать точные реплики поли-3-оксибутирата, которые характеризуются высокой степенью однородности по всей площади и отсутствием дефектных областей. Показано, что сформированные микроструктурированные пленки поли-3-оксибутирата с регулируемой топографией поверхности перспективны для использования в качестве скаффолдов для культивирования стволовых клеток.

The possibility of fabrication of microstructured poly-3-hydroxybutyrate films by self-assembly water microdroplets technique, using artificial templates and polymer inverse emulsions has been studied. It has been established that self-assembly water microdroplets technique allows forming ordered microstructures of poly-3-hydroxybutyrate with a hexagonal arrangement of cells with an adjustable diameter from 1 to 4 цт. It has been shown that application of inverse emulsions of poly-3-hydroxybutyrate allows us to fabricate porous films with a pore size in the range from 0.4 to 3 ^m, while the structure of the films and the pore size can be controlled by changing the polymer concentration in the dispersion medium and the volume ratio of the phases. Using spin-coating technique and artificial templates, it is possible to obtain poly-3-hydroxybutyrate microstructured replicas, which are characterized by a high degree of uniformity and the absence of defective areas. It has been shown that the formed microstructured poly-3-hydroxybutyrate films with controlled surface topography are promising for use as scaffolds for stem cells.

поли-3-оксибутиратпленкитопографиясамоорганизацияшаблонстволовые клеткиpoly-3-hydroxybutyratefilmstopographyself-organizationtemplatestem cellsРабота выполнена при поддержке БРФФИ (грант № Х20Р-165) и РФФИ (грант № 20-54-00021) (в части получения очищенного поли-3-оксибутирата и культивирования МСК на структурированных пленках) и в рамках государственного задания Министерства науки и высшего образования РФ (в части биосинтеза поли-3-оксибутирата). В работе использовано оборудование ЦКП «Электронная микроскопия в науках о жизни» МГУ им. М. В. Ломоносова (уникальное оборудование «Трехмерная электронная микроскопия и спектроскопия») и ФИЦ биотехнологии РАН.This work has been supported by the Belarusian Republican Foundation for Fundamental Research, grant No. Х20Р-165 and the Russian Foundation for Fundamental Research, grant No. 20-54-00021 (in terms of obtaining purified poly-3-hydroxybutyrate and cultivating MSCs on structured films) and within the framework of the state task of the Ministry of Science and Higher Education of the Russian Federation (in terms of the biosynthesis of po-ly-3-hydroxybutyrate). The work used the equipment of the Center for Collective Use “Electron Microscopy in the Life Sciences” of Lomonosov Moscow State University (unique equipment “Three-dimensional electron microscopy and spectroscopy”) and FRC Biotechnology RAS.ReferencesAdditive manufacturing techniques for the production of tissue engineering constructs / C. 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The authors declare that there are no conflicts of interest present.