vestichИзвестия Национальной академии наук Беларуси. Серия химических наукProceedings of the National Academy of Sciences of Belarus, Chemical Series1561-83312524-2342The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8331-2023-59-2-139-149vestich-812Research ArticleАНАЛИТИЧЕСКАЯ ХИМИЯANALYTICAL CHEMISTRYЭкстракция амитраза и его метаболитов органическими растворителямиExtraction of amitraz and its metabolites with organic solventsПолоневичА. Г.PolonevichA. G

Полоневич Анна Геннадьевна – соискатель кафедрыаналитической химии; ведущий химик 

ул. Ленинградская, 14, 220030, Минск; ул. Академическая, 8, 220012, Минск

 

Anna G. Polonevich – doctoral student at the Department of Analytical Chemistry; Leading Chemist

14, Leningradskaya Str., 220030; 8, Akademicheskaya Str.,220012, Minsk

gannapalanevich@gmail.comЛещевС. М.LeschevS. M.

Лещев Сергей Михайлович – д-р хим. наук, профессор

ул. Ленинградская, 14, 220030, Минск

Sergey M. Leschev – Ph. D. (Chemistry), Professor

14, Leningradskaya Str., 220030

БулгаковаО. А.BulhakavaV. A.

Булгакова Ольга Анатольевна – химик

Академическая, 8, 220012, Минск

Volha A. Bulhakava – chemist

8, Akademicheskaya Str., 220012, Minsk

БелышеваЛ. Л.BelyshevaL. L.

Белышева Людмила Леонидовна – зав. лаб.

Академическая, 8, 220012, Минск

Liudmila L. Belysheva – Head of the Laboratory

8, Akademicheskaya Str., 220012, Minsk

Белорусский государственный университет; Научно-практический центр гигиеныBelarusian State University; Scientific Practical Center of HygieneБелорусский государственный университетBelarusian State UniversityНаучно-практический центр гигиеныScientific Practical Center of Hygiene202305062023592139149Copyright © Полоневич А.Г., Лещев С.М., Булгакова О.А., Белышева Л.Л., 20232023Полоневич А.Г., Лещев С.М., Булгакова О.А., Белышева Л.Л.Polonevich A.G., Leschev S.M., Bulhakava V.A., Belysheva L.L.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestichem.belnauka.by/jour/article/view/812

Оценены константы распределения амитраза и трех его основных метаболитов (ДМФФ, ДМФ, ДМА) для экстракционных систем вода–органический растворитель (н-гексан, толуол, дихлорметан, хлороформ). Согласно анализу распределения веществ в изученных системах установлено, что дихлорметан является оптимальным экстрагентом для разработки методики одновременного определения амитраза и трех его метаболитов в меде, основанной на жидкость-жидкостной экстракции. На основании изучения стабильности амитраза в водно-ацетонитрильных растворах с различными добавками установлен состав растворителя для итоговых растворов проб – 50 мМ раствор аммиака в смеси вода–ацетонитрил (1 : 1, об.). 

The distribution constants of amitraz and its three main metabolites (DMFF, DMF, DMA) for water–organic solvent (n-hexane, toluene, dichloromethane, chloroform) extraction systems were estimated. Based on the analysis of the substances distribution in the studied systems, it was established that dichloromethane is the optimal choice for amitraz and its metabolites liquid-liquid extraction for method development for their simultaneous determination in honey. Amitraz stability study in water-acetonitrile solutions with various additives demonstrated that 50 mM ammonia solution in water– acetonitrile mixture (1 : 1, vol.) should be used as solvent for the samples final solutions. 

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The authors declare that there are no conflicts of interest present.