vestichИзвестия Национальной академии наук Беларуси. Серия химических наукProceedings of the National Academy of Sciences of Belarus, Chemical Series1561-83312524-2342The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8331-2023-59-3-225-233vestich-828Research ArticleБИООРГАНИЧЕСКАЯ ХИМИЯBIOORGANIC CHEMISTRYЭкспрессия и очистка рекомбинантного термостабильного ДНК-связывающего белка Sso7dExpression and purification of recombinant thermostable DNA-binding protein Sso7dСаченкоА. Б.SachankaA. B.

Саченко Антон Борисович – мл. науч. сотрудник, аспирант

ул. Купревича, 5/2, 220141, Минск

Sachanka Antos B. – Junior Researcher, Postgraduate Student

5/2, Kuprevich Str., 220141, Minsk

antosuk@yandex.ruТравкинаМ.TrawkinaM.

Травкина Мария – науч. сотрудник

ул. Купревича, 5/2, 220141, Минск

Trawkina Maria – Researcher

5/2, Kuprevich Str., 220141, Minsk

mtrawkina@gmail.comЩурВ. В.ShchurV. V.

Щур Вероника Владимировна – науч. сотрудник

ул. Купревича, 5/2, 220141, Минск

Shchur Veronika V. – Researcher

5/2, Kuprevich Str., 220141, Minsk

shchur931@gmail.comУсановC. А.UsanovS. A.

Усанов Сергей Александрович – член-корреспондент, д-р хим. наук

ул. Купревича, 5/2, 220141, Минск

Usanov Sergei A. – Corresponding Member of the National Academy of Sciences of Belarus, D. Sc. (Chemistry), Professor

5/2, Kuprevich Str., 220141, Minsk

usanov@iboch.byЯнцевичА. В.YantsevichA. V.

Янцевич Алексей Викторович – канд. хим. наук, доцент, зав. лаб

ул. Купревича, 5/2, 220141, Минск

Yantsevich Aliaksei V. – Ph. D. (Chemistry), Associate Professor, Head of the Laboratory

5/2, Kuprevich Str., 220141, Minsk

al.yantsevich@gmail.comИнститут биоорганической химии Национальной академии наук БеларусиInstitute of Bioorganic Chemistry of the National Academy of Sciences of Belarus202304092023593225233Copyright © Саченко А.Б., Травкина М., Щур В.В., Усанов C.А., Янцевич А.В., 20232023Саченко А.Б., Травкина М., Щур В.В., Усанов C.А., Янцевич А.В.Sachanka A.B., Trawkina M., Shchur V.V., Usanov S.A., Yantsevich A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestichem.belnauka.by/jour/article/view/828

Белок Sso7d обладает исключительной стабильностью структуры, а также способностью высокоспецифично связываться с ДНК, что делает белок перспективным модулем для создания химерных белков и тест-систем. Sso7d является компонентом химерных высокоточных ДНК-полимераз, способных осуществлять полимеразную цепную реакцию даже в присутствии ингибиторов ПЦР. Применение более быстрого, простого и высокопроизводительного метода получения белка позволит существенно сократить расходы на создание биосенсоров и на проведение анализов. Описан новый эффективный способ получения рекомбинантного белка Sso7d с высокой степенью чистоты без использования аффинной хроматографии.

The Sso7d protein has exceptional structural stability and the ability to bind highly specifically to DNA, which makes the protein a promising module for creating fusion proteins and test systems. Sso7d is a part of fusion high-fidelity DNA polymerases capable of carrying out the polymerase chain reaction even in the presence of PCR inhibitors. Application of faster, simpler, and more efficient method for protein production will significantly reduce the cost of creating biosensors and conducting analyzes. This paper describes a new efficient method for obtaining recombinant Sso7d protein with a high degree of purity without using affinity chromatography.

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The authors declare that there are no conflicts of interest present.