vestichИзвестия Национальной академии наук Беларуси. Серия химических наукProceedings of the National Academy of Sciences of Belarus, Chemical Series1561-83312524-2342The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8331-2023-59-4-302-311vestich-848Research ArticleАНАЛИТИЧЕСКАЯ ХИМИЯANALYTICAL CHEMISTRYЭкстракция анаболических стероидов органическими растворителями из водных растворовOrganic solvent extraction of anabolic steroids from aqueous solutionshttps://orcid.org/0000-0001-5378-1718ЛещевС. М.LeschevS. M.

Лещев Сергей Михайлович – доктор химических наук, профессор

ул. Ленинградская, 14, 220030, Минск

Leschev Sergey M. – D. Sc. (Chemistry), Professor

14, Leningradskaya Str., 220030, Minsk

leschev.sergey54@gmail.comhttps://orcid.org/0000-0002-7972-7784ПоходняЮ. Г.PakhadniaYu. G.

Походня Юрий Георгиевич – кандидат биологических наук, директор

аг. Лесной, 31, 223040, Минская область

Pakhadnia Yury G. – Ph. D. (Biology), Director

31, Liasny, 223040, Minsk region

director@antidoping.byhttps://orcid.org/0000-0002-8293-5840ЧеховскаяО. Н.TchekhovskayaO. N.

Чеховская Ольга Николаевна – ведущий химик

аг. Лесной, 31, 223040, Минская область

Tchekhovskaya Olga N. – Leading Chemist

31, Liasny, 223040, Minsk region

testing@antidoping.byhttps://orcid.org/0000-0003-3201-3511АгабалаевА. А.AhabalayeuA. A.

Агабалаев Александр Андреевич – кандидат химических наук, заместитель заведующего лабораторией

пр-т Дзержинского, 83/15, 220045, Минск

Ahabalayeu Aliaksandr A. – Ph. D. (Chemistry). Deputy Head of the Laboratory

83/15, Dzerzhinsky Ave., 220045, Minsk

alexandrmailbox@inbox.ruhttps://orcid.org/0000-0002-8400-6359ЗаяцМ. Ф.ZayatsM. F.

Заяц Михаил Федорович – доктор химических наук, заведующий кафедрой

ул. Ленинградская, 14, 220030, Минск

Zayats Mikhail F. – D. Sc. (Chemistry), Head of the Department

14, Leningradskaya Str., 220030, Minsk

mikhail_zayats@tut.byБелорусский государственный университетBelarusian State UniversityНациональная антидопинговая лабораторияNational Anti-Doping LaboratoryЦентр экспертиз и испытаний в здравоохраненииCenter for Examinations and Tests in Health Service202302122023594302311Copyright © Лещев С.М., Походня Ю.Г., Чеховская О.Н., Агабалаев А.А., Заяц М.Ф., 20232023Лещев С.М., Походня Ю.Г., Чеховская О.Н., Агабалаев А.А., Заяц М.Ф.Leschev S.M., Pakhadnia Y.G., Tchekhovskaya O.N., Ahabalayeu A.A., Zayats M.F.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestichem.belnauka.by/jour/article/view/848

При 20 °С изучена экстракция ряда анаболических стероидов (тестостерон; эпитестостерон; эпиметендиол; 17α-метилтестостерон; 19-норэтиохоланолон; 18-норметенол; 19-норандростерон; 3α-гидрокси-2α-метил-5α-андростан-17-он; 9α-фтор-17,17-диметил-18-норандростан-4,13-диен-11β-ол-3-oн; 1α-метил-5α-андростан-3αол-17-он; 1α-метил-5α-андростан-3α,17β-диол; эпиоксандролон; метастерон; оксандролон; форместан; 16β-гидроксифуразабол) органическими растворителями из воды и водно-солевых растворов. На основании полученных экспериментальных данных рассчитаны константы распределения анаболических стероидов (АС), которые были использованы для оптимизации стандартной методики пробоподготовки в процессе определения анаболических андрогенных стероидов (ААС) и их метаболитов в моче спортсменов. Установлено, что наиболее селективным экстрагентом из водных и водно-солевых растворов является гексан, извлекающий большинство ААС. Для повышения степени экстракции плохо извлекаемых гексаном ААС целесообразно применять высаливание сульфатом натрия или аммония и использовать более активные органические экстрагенты – хлористый метилен или диэтиловый эфир. Разработана экстракционная методика пробоподготовки мочи спортсменов для последующего определения в ней анаболических стероидов и их метаболитов методом газовой хроматографии с масс-спектрометрическим детекторованием. Предложенная методика характеризуется стандартным отклонением 10–15 % и пределом обнаружения около 10 нг/мл мочи.

The extraction of anabolic steroids (testosterone; epitestosterone; epimethenediol; 17α-methyltestosterone; 19-norethiocholanolone; 18-normethenol; 19-norandrosterone; 3α-hydroxy-2α-methyl-5α-androstan-17-one; 9α-fluoro-17,17dimethyl-18-norandrostan-4,13-dien-11β-ol-3-one; 1α-methyl-5α-androstan-3α-ol-17-one; 1α-methyl-5αandrostan-3α,17β-diol; epioxandrolone; methasterone; oxandrolone; formestane; 16β-hydroxyfurazabol) with organic solvents from water and aqueous salt solutions was studied at 20 ° C. Based on the experimental data obtained, the partition ratios of anabolic steroids (AS) were calculated, which were used to optimize the standard sample preparation procedure in the process of determining anabolic androgenic steroids (AAS) and their metabolites in the urine of athletes. It was found that the most selective extractant from aqueous and aqueous salt solutions is hexane, which extracts the majority of AAS. To increase the recovery of AAS, poorly extracted by hexane, it is advisable to use salting out with sodium or ammonium sulfate and use more active organic extractants – methylene chloride or diethyl ether. An extraction sample preparation technique has been developed for the subsequent determination of anabolic steroids and their metabolites in athletes’ urine by gas chromatography with mass spectrometric detection method. The proposed method is characterized by a standard deviation of 10 – 15 % and a detection limit of about 10 ng/ml of urine.

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The authors declare that there are no conflicts of interest present.