vestichИзвестия Национальной академии наук Беларуси. Серия химических наукProceedings of the National Academy of Sciences of Belarus, Chemical Series1561-83312524-2342The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8331-2024-60-1-36-44vestich-860Research ArticleБИООРГАНИЧЕСКАЯ ХИМИЯBIOORGANIC CHEMISTRYCинтез тиолсодержащих ДНК-олигонуклеотидов с использованием фосфорамидитного реагента на основе транс-4-гидрокси-L-пролинолаSynthesis of thiol-containing DNA-oligonucleotides using a phosphoramidite reagent based on trans -4-hydroxy-L-prolinolЛамекинаЮ. П.LamekinaY. P.

Ламекина Юлия Петровна – научный cотрудник.

ул. Сурганова, 13, 220072, Минск

Yuliya P. Lamekina – Researcher.

13, Surganov Str., 220072, Minsk

yplamekina@gmail.comСеверинчикТ. П.SeviarynchykT. P.

Северинчик Татьяна Петровна – научный cотрудник.

ул. Сурганова, 13, 220072, Минск

Tatsiana P. Seviarynchyk – Researcher.

13, Surganov Str., 220072, Minsk

tanyaseverinchik@gmail.comУлащикЕ. А.UlashchikE. A.

Улащик Егор Александрович – научный cотрудник.

ул. Сурганова, 13, 220072, Минск

Egor A. Ulashchik – Researcher.

13, Surganov Str., 220072, Minsk

ulaschick@gmail.comАхламёнокТ. П.AkhlamionokT. P.

Ахламёнок Татьяна Петровна – младший научный cотрудник.

ул. Сурганова, 13, 220072, Минск

Tatsiana P. Akhlamionok – Junior Researcher.

13, Surganov Str., 220072, Minsk

t.akhlamionak@gmail.comБорищикП. Ю.BaryshchykP. Y.

Борищик Полина Юрьевна – младший научный cотрудник.

ул. Сурганова, 13, 220072, Минск

Palina Y. Baryshchyk – Junior Researcher.

13, Surganov Str., 220072, Minsk

contact.polina@yandex.byРонишенкоБ. В.RanishenkaB. V.

Ронишенко Богдан Вячеславович – кандидат химических наук, научный cотрудник.

ул. Сурганова, 13, 220072, Минск

Bahdan V. Ranishenka – Ph. D. (Chemistry), Researcher.

13, Surganov Str., 220072, Minsk

ranishenka@gmail.comШманайВ. В.ShmanaiV. V.

Шманай Вадим Владимирович – кандидат химических наук, заведующий лабораторией.

ул. Сурганова, 13, 220072, Минск

Vadim V. Shmanai – Ph. D. (Chemistry), Head of the Laboratory.

13, Surganov Str., 220072, Minsk

shmanai@ifoch.bas-net.byИнститут физико-органической химии Национальной академии наук БеларусиInstitute of Physical Organic Chemistry of the National Academy of Sciences of Belarus2024040320246013644Copyright © Ламекина Ю.П., Северинчик Т.П., Улащик Е.А., Ахламёнок Т.П., Борищик П.Ю., Ронишенко Б.В., Шманай В.В., 20242024Ламекина Ю.П., Северинчик Т.П., Улащик Е.А., Ахламёнок Т.П., Борищик П.Ю., Ронишенко Б.В., Шманай В.В.Lamekina Y.P., Seviarynchyk T.P., Ulashchik E.A., Akhlamionok T.P., Baryshchyk P.Y., Ranishenka B.V., Shmanai V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestichem.belnauka.by/jour/article/view/860

Осуществлен синтез нового фосфорамидитного реагента на основе транс-4-гидрокси-L-пролинола для получения тиолсодержащих олигонуклеотидов. Реакционная способность введенных тиольных групп была продемонстрирована на примере реакций с тиол-специфичными реагентами. Конъюгаты после модификации были очищены гель-фильтрацией и охарактеризованы с помощью ВЭЖХ-МС.

In this work, a new phosphoramidite reagent for the preparation of thiol-modified oligonucleotides was synthesized. Thiol-specific reagents have been used to demonstrate the reactivity of thiol groups. After modification, conjugates were purified via gel-filtration and characterized with HPLC-MS.

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The authors declare that there are no conflicts of interest present.