vestichИзвестия Национальной академии наук Беларуси. Серия химических наукProceedings of the National Academy of Sciences of Belarus, Chemical Series1561-83312524-2342The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8331-2024-60-1-45-52vestich-861Research ArticleХИМИЯ ВЫСОКОМОЛЕКУЛЯРНЫХ СОЕДИНЕНИЙPOLYMER CHEMISTRYЭксклюзионная жидкостная хроматография карбоксиметилхитина и карбоксиметилхитозана из рачков Artemia Parthenogenetica Аральского моряExclusion liquid chromatography of carboxymethylchitin and carboxymethylchitosan from crustacean Artemia Parthenogenetica of the Aral SeaБоймирзаевА. С.BoymirzaevA. S.

Боймирзаев Азамат Солиевич – доктор химических наук, профессор.

ул. Касансай, 7, 160115, Наманган

Azamat S. Boymirzaev – D. Sc. (Chemistry), Professor, Namangan Institute of Engineering and Technology.

7, Kasansay Str., Namangan

a.boymirzayev@nammti.uzБердимбетоваГ. Е.BerdimbetovaG. E.

Бердимбетова Гулсара Есеновна – кандидат химических наук, старший научный сотрудник.

ул. Бердак, 41, Нукус, Республика Каракалпакстан

Gulsara Y. Berdimbetova – Ph. D. (Chemistry), Senior Researcher, Karakalpak Scientific Research Institute of Natural Sciences.

41, Berdak Str., Nukus, Republic of Karakalpakstan

gulsara2@rambler.ruКарлыбаеваБ. П.KarlybaevaB. P.

Карлыбаева Бахтыгул Пердебаевна – докторант.

ул. Бердак, 41, Нукус, Республика Каракалпакстан

Bakhtygul P. Karlybaeva – Doctoral Student, Karakalpak Scientific Research Institute of Natural Sciences.

41, Berdak Str., Nukus, Republic of Karakalpakstan

bqarlibaeva@inbox.ruНаманганский инженерно-технологический институтNamangan Institute of Engineering and TechnologyКаракалпакский научно-исследовательский институт естественных наук, Каракалпакское отделение Академии наук Республики УзбекистанKarakalpak Research Institute of Natural Sciences, Karakalpak Branch of the Academy of Sciences of the Republic of Uzbekistan2024040320246014552Copyright © Боймирзаев А.С., Бердимбетова Г.Е., Карлыбаева Б.П., 20242024Боймирзаев А.С., Бердимбетова Г.Е., Карлыбаева Б.П.Boymirzaev A.S., Berdimbetova G.E., Karlybaeva B.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestichem.belnauka.by/jour/article/view/861

Разработаны методики получения водорастворимых производных хитина и хитозана из цист рачков рода Artemia Parthenogenetica Аральского моря путем карбоксиметилирования ОН- и NH2-групп их цепей. Изучены элюционные, вязкостные и полиэлектролитные свойства карбоксиметилхитина и карбоксиметилхитозана в эксклюзионной жидкостной хроматографии. Подавление электростатических эффектов и реализация эксклюзионного механизма разделения указанных полисахаридов достигнуто при использовании в качестве элюента водного раствора нитрата натрия с концентрацией 0,1 моль/л, который и был рекомендован для определения молекулярно-массовых характеристик.

The article deals with the synthesis of water-soluble derivatives of chitin and chitosan from cysts of crustaceans of the genus Artemia Parthenogenetica of the Aral Sea by carboxymethylation of OH and NH2 groups of their chains. The elution, viscosity and polyelectrolyte properties in size exclusion liquid chromatography of carboxymethylchitin and carboxymethylchitosan were studied. The suppression of electrostatic effects and the implementation of the exclusion mechanism for the separation of these polysaccharides were achieved by using an aqueous solution of sodium nitrate with a concentration of 0.1 mol/l as an eluent, which was recommended for determining molecular weight characteristics.

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The authors declare that there are no conflicts of interest present.