vestichИзвестия Национальной академии наук Беларуси. Серия химических наукProceedings of the National Academy of Sciences of Belarus, Chemical Series1561-83312524-2342The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8331-2025-61-2-95-104vestich-948Research ArticleФИЗИЧЕСКАЯ ХИМИЯPHYSICAL CHEMISTRYФизико-химические свойства новых производных цианиновых красителей в составе конъюгатов с ДНКPhysicochemical properties of new cyanine dye derivatives in DNA conjugatesФаньФ.FanF.

Фань Фань – аспирант.

ул. Сурганова, 13, 220072, Минск

Fan Fan – Postgraduate Student, Institute of Physical-Organic Chemistry of the National Academy of Science of Belarus.

13, Surganov Str., 220072, Minsk

zwth6230@gmail.comПоведайлоВ. А.PovedailoV. A.

Поведайло Владимир Александрович – доктор физико-математических наук, главный научный сотрудник.

пр-т Независимости, 68, 220072, Минск

Vladimir A. Povedailo – Dr. Sc. (Physics and Mathematics), Chief Researcher, Institute of Physics of the National Academy of Science of Belarus.

68, Nezavisimosti Ave., 220072, Minsk

poved@dragon.bas-net.byКадуцкийА. П.KadutskiiA. P.

Кадуцкий Алексей Петрович – научный сотрудник.

ул. Сурганова, 13, 220072, Минск

Aleksey P. Kadutskii – Researcher, Institute of Physical-Organic Chemistry of the National Academy of Science of Belarus.

13, Surganov Str., 220072, Minsk

kadutskii@gmail.comМалеевГ. В.MaleevG. V.

Малеев Григорий Владимирович – старший научный сотрудник ИФАВ РАН.

пр-т академика Семенова, 1, 142432, Черноголовка

Grigoriy V. Maleev – Senior Researcher, Institute of Physiologically Active Compounds at Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry of Russian Academy of Sciences.

1, Academian Semenov Ave., Chernogolovka, Moscow region, 142432

g.maleev@ipac.ac.ruШманайВ. В.ShmanaiV. V.

Шманай Вадим Владимирович – кандидат химических наук, доцент, заведующий лаборатории.

ул. Сурганова, 13, 220072, Минск

Vadim V. Shmanai – Ph. D. (Chemistry), Associate Professor, Head of the Laboratory, Institute of Physical-Organic Chemistry of the National Academy of Science of Belarus.

13, Surganov Str., 220072, Minsk

v.shmanai@gmail.comИнститут физико-органической химии Национальной академии наук БеларусиInstitute of Physical Organic Chemistry, National Academy of Sciences of BelarusИнститут физики имени Б.И. Степанова Национальной академии наук БеларусиB.I. Stepanov Institute of Physics, National Academy of Sciences of BelarusИнститут физиологически активных веществ Федерального исследовательского центра проблем химической физики и медицинской химии Российской академии наук (ИФАВ РАН)Institute of Physiologically Active Compounds at Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry of Russian Academy of Sciences20252905202561295104Copyright © Фань Ф., Поведайло В.А., Кадуцкий А.П., Малеев Г.В., Шманай В.В., 20252025Фань Ф., Поведайло В.А., Кадуцкий А.П., Малеев Г.В., Шманай В.В.Fan F., Povedailo V.A., Kadutskii A.P., Maleev G.V., Shmanai V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestichem.belnauka.by/jour/article/view/948

Цианиновые красители – один из наиболее часто используемых классов флуоресцентных красителей. Все Cy5 флуоресцируют на длине волны около 660 нм, а Cy7 – в ближнем инфракрасном диапазоне длин волн (700–900 нм), что делает их предпочтительными для биологических исследований, так как в этой области почти отсутствует фоновая флуоресценция клеток. Интенсивность флуоресценции цианиновых красителей часто изменяется после конъюгации с биомолекулами, в том числе с нуклеиновыми кислотами. Кроме того, она может существенно изменяться в результате образования дуплекса модифицированной красителем одноцепочечной ДНК с комплементарной последовательностью. Исследованы физико-химические свойства ряда производных красителей Cy5 и Cy7 с заместителями разной длины в различных положениях молекул в составе конъюгатов с одноцепочечной и двухцепочечной ДНК.

Cyanine dyes are one of the most commonly used classes of fluorescent probes. All Cy5 fluoresce at ~ 660 nm, while Cy7 emit in the near-infrared range (700–900 nm), making them particularly suitable for biomedical applications due to reduced tissue autofluorescence in this spectral region. The fluorescence intensity of cyanine dyes typically increases upon conjugation with biomolecules such as nucleic acids. Furthermore, their fluorescence can be significantly modulated through duplex formation between dye-modified single-stranded DNA and its complementary sequence. In this study, we investigated physicochemical properties of a series of Cy5 and Cy7 derivatives with substituents of varying lengths at distinct molecular positions in both single-and double-stranded DNA conjugates.

цианиновые красителиДНКквантовый выходфлуоресценцияклик-реакцияcyanine dyesDNAquantum yieldfluorescenceclick-reactionРабота выполнена при поддержке Белорусского республиканского фонда фундаментальных исследований (проекты № Ф24В-006 и Х23РНФ-041) и фонда Китайского стипендиального совета (№ 202008400011). Авторы выражают благодарность М.Р. Ребковец и В.В. Каминской за синтез исследованных олигонуклеотидов.This work has been performed with the financial support of Belarusian Republican Foundation for Fundamental Research (projects No. Ф24В-006 and Х23РНФ-041) and China Scholarship Council Foundation (No. 202008400011). The authors express their gratitude to M.R. Rebkovets and V.V. Kaminskaya for the synthesis of the studied oligonucleotides.ReferencesTemperature dependence of interaction between double stranded DNA and Cy3 or Cy5 / X. Li, Y. Yin, X. 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The authors declare that there are no conflicts of interest present.