vestichИзвестия Национальной академии наук Беларуси. Серия химических наукProceedings of the National Academy of Sciences of Belarus, Chemical Series1561-83312524-2342The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"vestich-99Research ArticleБИООРГАНИЧЕСКАЯ ХИМИЯBIOORGANIC CHEMISTRYГетерологическая экспрессия, выделение и физико-химические свойства рекомбинантного цитохрома CYP1B1 человекаHeterologous expression, isolation, purification, physical and chemical properties of recombinant human cytochrome CYP1B1ЛущикА. Я.LushchykA. J.noemail@neicon.ruГилепА. А.GilepA. A.noemail@neicon.ruГрабовецИ. П.GrabovetsI. P.noemail@neicon.ruУсановС. А.UsanovS. A.noemail@neicon.ruИнститут биоорганической химии НАН БеларусиBelarus201508062016017984Copyright © Лущик А.Я., Гилеп А.А., Грабовец И.П., Усанов С.А., 20162016Лущик А.Я., Гилеп А.А., Грабовец И.П., Усанов С.А.Lushchyk A.J., Gilep A.A., Grabovets I.P., Usanov S.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestichem.belnauka.by/jour/article/view/99

С целью изучения каталитических свойств СYP1B1 по отношению к ряду стероидных соединений нами разработана эффективная система гетерологической экспрессиии гемопротеида в E.coli и выделен в высокоочищенном состоянии «растворимый» препарат рекомбинантного CYP1B1. Установлены параметры взаимодействия данного фермермента с стероидными лигандами.

In order to study the catalytic properties of CYP1B1 toward a number of steroid compounds, we have developed an effective system of heterologous expression of hemoprotein in E.coli and isolated the preparation of recombinant CYP1B1 «soluble» form in a highly purified state. The interaction parameters of the purified enzyme with steroid ligands have been determined.

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The authors declare that there are no conflicts of interest present.