MOLECULAR CLONING, HETEROLOGICAL EXPRESSION AND PRODUCTION OF THE RECOMBINANT G. GALLUS GROWTH
Abstract
Growth hormones (GH) are polypeptides that trigger different biochemical pathways. GH main function is to regulate anabolic and catabolic processes. GH also plays an important role as immunomodulator. In this paper, molecular cloning, heterological expression and purification of the recombinant G. gallus growth hormone have been conducted. Two biotechnological schemes have been developed: cytosol expression followed by inclusion bodies solubilisation and periplasmatic secretion. Expression levels of 2.43 and 15 mg per 1 liter of bacterial culture respectively were achieved. It has been shown that the best way of expression is periplasmatic secretion, which does not require complex solubilisation and refolding procedures and results in higher product yield and purity.
About the Authors
A. M. Gorkavaya
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
G. V. Sergeev
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
A. A. Gilep
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
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