NMR INVESTIGATION OF THE COMPOSITION OF AQUEOUS EXTRACTS FROM POT MARIGOLD INFLORESCENCE

Using NMR analysis, the composition of the aqueous extracts of pot marigold inflorescence of cultivars “Geisha”, “Mahrovy 2000”, “Dragon”, “Prince of India” were investigated. Inflorescences of pot marigold were used for the analysis. Plants were grown in the experimental area of the Department of Biochemistry and Biotechnology of Plants of Central Botanical Garden of the NAS of Belarus. Inflorescence of “Mahrovy 2000” was collected in July 2013–2016, other varieties – in 2016. The raw materials were air dried and kept in the dark, then crushed in an agate mortar or in a coffee grinder. The extraction was performed with distilled and deuterated water (D₂O) at a ratio of plant raw material : extractant = 1 : 15. As a result, the qualitative and quantitative composition of aqueous extracts of cultivars was determined. In the investigated samples 11 aminoacids: alanine, γ-aminobutyric acid, asparagine, valine, glutamine, isoleucine, leucine, proline, tyrosine, threonine and phenylalanine were determined, asparagine and proline prevailed. Monosaccharides, such as 2 isoforms of glucose and 3 isoforms of fructose, were determined as well. Analysis of the spectra on 31Р showed that phosphate is present in significant amounts in the aqueous extract of pot marigold flowers. “Geisha”, “Mahrovy 2000” and “Prince of India” varieties were shown to have similar qualitative and quantitative composition of carbohydrates and sugars. Spectrum of extracts of the variety “Dragon” was different from the others with the asparagine predomination in the pool of amino acids, and the content of β-fructopyranose lower in comparison with other varieties.

1. Muney B. P., Khadabadi S. S., Banarase N. B., “Phytochemical Compostituents and Pharmacological Activities of Calendula officinalis Linn (Asteraceae): A Review”, Tropical Jornal of Pharmaceutical Research, 2009, vol. 8(5), pp. 455–465.

2. “Specialized cadastre of plants, animals, mushrooms”, Available at: http://bchemcad.basnet.by/plants.php, (accessed 25.08.2016).

3. Agabaliaeva E. D., Karankevich E. G., Matveichuk S. V., Reshetnikov V. N., Skakovskii E. D., Tychinskaia L. Iu., “NMR spectroscopy of aqueous extracts of fenugreek (Trigonella foenum graecum L.)”, Zhurnal prikladnoi spektroskopii [Journal of Applied Spectroscopy (JAS)], 2014, vol. 81, no. 4, pp. 543–547.

4. Barclay T., Ginic-Markovic M., Johnston M. R., Cooper P., Petrovsky N., “Obcervation of the keto tautomer of d-fructose in D2O using 1H NMR spectroscopy”, Carbohydrate research, 2012, vol. 347, no. 1, pp. 136–141.

5. Curatolo W., Neuringer L. J., Ruben D., Haberkorn R., “Two-dimensional J-resolved 1H-nuclear magnetic resonance spectroscopy of α,β-d-glucose at 500 MHz”, Carbohydrate research, 1983, vol. 112, no. 2, pp. 297–300.

6. Yamaji, T. “Welcome to Spectral Database for Organic Compounds, SDBS”, Available at: http://sdbs.db.aist.go.jp, (accessed Feb.25, 2016).

7. Safonova I. A., “A study of the amino acid composition of the Pacific ninebark (Physocarpus opulifolius (L.) Maxim)”, Available at: http://cyberleninka.ru/article/n/izuchenie-aminokislotnogo-sostava-puzyreplodnika-kalinolistnogo-physocarpusopulifolius-l-maxim, (accessed 25.08.2016).

8. Ismagilov R. R., Kostylev D. A., Kalendula [Calendula], BGAU, Ufa, RU, 2000.