PREPARATION OF PROTEIN CONJUGATES AND MONOCLONAL ANTIBODIES FOR IMMUNOASSAY OF TETRACYCLINE
https://doi.org/10.29235/1561-8331-2018-54-1-72-79
Abstract
We report herein on the methods to introduce a desirable number of modifications into bovine serum albumin and horseradish peroxidase in a controlled manner. The low modification to protein ratio is required when the biological activity of the enzyme has to be preserved, and the high modification to protein ratio is needed when producing immunogens. By varying the linker and the site of antigen binding with the carrier protein, we were able to produce the antibodies of high specificity. Protein conjugates of tetracycline were synthesized and characterized. Immunization of mice was carried out and monoclonal antibodies for immunoassay of tetracycline were obtained and tested in ELISA, IC50=5 ug/ml.
Supporting agencies: We thank Dr. Christoph Weise (Freie Universität Berlin) for assistance in obtaining and interpreting the mass-spectra.
About the Authors
A. S. Kruhlik
Institute of Physical Organic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Researcher
13, Surganov Str., 220072, Minsk
S. T. Akalovich
Institute of Physical Organic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Ph. D. (Biology), Senior researcher, Research and Production Center for Hematology and Medical Biotechnology
160, Dolginovski tract, 220053, Minsk
O. L. Sharko
The Republic Research & Production Center for Transfusiology and Medical Biotechnologies
Belarus
Belarus
Ph. D. (Chemistry), Senior researcher
13, Surganov Str., 220072, Minsk
V. V. Shmanai
Institute of Physical Organic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Ph. D. (Chemistry), Head of the Laboratory of Bioconjugate Chemistry
13, Surganov Str., 220072, Minsk
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