Molecular cloning, heterological expression and production of the human growth hormone
https://doi.org/10.29235/1561-8331-2019-55-2-182-187
Abstract
Growth hormone (GH) is a polypeptide produced in the anterior pituitary lobe that triggers different biochemical pathways and increases cell proliferation and growth. In this work, the pNic based vector for periplasmatic secretion was developed. Recombinant human growth hormone was produced via periplasmatic secretion (42h, 30 °С) followed by several steps of purification. GH obtained does not contain His-tag. Expression level of 2,85 mg per1 literof bacterial culture was achieved.
About the Authors
A. M. Gorkavaya
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Anna M. Gorkavaya – Researcher
5/2, Kuprevich Str., 220141, Minsk
A. A. Gilep
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Andrei A. Gilep – Ph. D. (Chemistry), Leading researcher
5/2, Kuprevich Str., 220141, Minsk
G. V. Sergeev
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Gennady V. Sergeev – Ph. D. (Chemistry), Head of the laboratory
5/2, Kuprevich Str., 220141, Minsk
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