Selectivity of affinity sorbents based on aromatic peptides for the binding of class G immunoglobulins
https://doi.org/10.29235/1561-8331-2020-56-3-333-338
Abstract
Biospecific sorbents for the removal of IgG and subclasses from biological fluids based on oligopeptides that contain aromatic protein residues have been created. A functional assessment of high-quality experimental samples of sorbents and their preferences for IgG subclasses was carried out. It was found that each sorbent has good characteristics for removing IgG from biological fluids, but the sorbent based on Phe-Trp-DTyr is more effective in binding of total IgG. With respect to IgG subclasses, the best binding results are as follows: Phe-Gln-Tyr-OMe - IgG1 (86,53%), Phe-Ala-Tyr - IgG2 (60,2%), Phe-Trp-DTyr - IgG3 (59,52%) and IgG4 (55,05%).
Keywords
sorption,
sorbents,
immunoglobulins,
subclasses,
proteins,
ligands,
peptides,
selectivity,
sorption capacity
About the Authors
Y. S. Pustsyulga
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus
Russian Federation
Russian Federation
Yegor S. Pustyulga - Researcher.
5/2, acad. V. F. Kuprevich Str., 220141, Minsk
O. V. Gribovskaya
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus
Russian Federation
Russian Federation
Olga V. Gribovskaya - Senior Researcher.
5/2, acad. V. F. Kuprevich Str., 220141, Minsk
E. M. Ermola
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus
Russian Federation
Russian Federation
Eugeny M. Ermola - Researcher.
5/2, acad. V. F. Kuprevich Str., 220141, Minsk
V. P. Golubovich
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus
Russian Federation
Russian Federation
Vladimir P. Golubovich - D. Sc. (Biology), Professor, Head of the Laboratory.
5/2, acad. V. F. Kuprevich Str., 220141, Minsk
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