Microspheres made of polyvinyl alcohol crosslinked with glutaraldehyde for immunomagnetic cell separation

K. V. Laznev; V. E. Agabekov

doi:10.29235/1561-8331-2020-56-4-391-398

Microspheres made of polyvinyl alcohol crosslinked with glutaraldehyde for immunomagnetic cell separation

K. V. Laznev, V. E. Agabekov

https://doi.org/10.29235/1561-8331-2020-56-4-391-398

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Abstract

Emulsification of water solutions of NaCl and CaCl₂ in isooctane with Span 85 and Tween 85 additives was found to depend on the salt content and the cation nature, Ca²⁺ being more effective stabilizer than Na+. When the solutions of 275 g/l glutaraldehyde with 1М CaCl₂ in water and 2.8 %m Span 85 with 1.5 %m Tween 85 in isooctane were mixed and sonicated, the transparent emulsion (turbidity <20 FNU) was acquired. It was used as а crosslinking agent to synthesize microspheres from magnetic fluids containing polyvinyl alcohol and magnetite nanoparticles dispersed as microdroplets in isooctane. The microspheres were modified with avidin and used for immunomagnetic separation of cells.

Keywords

microspheres, emulsions, polyvinyl alcohol, glutaraldehyde, immunomagnetic separation

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ISSN 1561-8331 (Print)
ISSN 2524-2342 (Online)

Proceedings of the National Academy of Sciences of Belarus, Chemical Series

Microspheres made of polyvinyl alcohol crosslinked with glutaraldehyde for immunomagnetic cell separation

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About the Authors

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Proceedings of the National Academy of Sciences of Belarus, Chemical Series

Microspheres made of polyvinyl alcohol crosslinked with glutaraldehyde for immunomagnetic cell separation

Full Text:

Abstract

Keywords

About the Authors

References

Review

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