Testing of experimental samples of sorbents based on aromatic peptides
https://doi.org/10.29235/1561-8331-2020-56-4-465-472
Abstract
By dynamic experiments, the purpose of which was to establish the time during which the saturation of sorbents occurs, as well as the maximum amount of total IgG bound, the saturation and capacity properties were revealed. Sorption dynamics data have been obtained for tripeptides: Phe-Trp-DTyr, Trp-Phe-DTyr, Phe-Ala-Tyr-OMe, Phe-Asn-Tyr-OMe, Phe-Asp (Bzl)-Tyr-OMe, Phe-Gln-Tyr-OMe and Phe-Gly-Tyr. Specific saturation was reliably confirmed for the Phe-Trp-DTyr sample and the Phe-Gln-Tyr-OMe sample. Other samples of sorbents show positive dynamics with episodic periods of desorption, with further alignment of the positive dynamics of the sorption process.
About the Authors
Y. S. Pustsyulga
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus
Russian Federation
Russian Federation
Yegor S. Pustyulga – Researcher.
5/2, Acad. V. F. Kuprevich Str., 220141, Minsk
O. V. Gribovskaya
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus
Russian Federation
Russian Federation
Olga V. Gribovskaya - Senior Researcher.
5/2, Acad. V. F. Kuprevich Str., 220141, Minsk
E. M. Ermola
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus
Russian Federation
Russian Federation
Eugeny M. Ermola - Researcher.
5/2, Acad. V. F. Kuprevich Str., 220141, Minsk
V. P. Golubovich
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus
Russian Federation
Russian Federation
Vladimir P. Golubovich - D. Sc. (Biology), Professor, Head of the Laboratory.
5/2, Acad. V. F. Kuprevich Str., 220141, Minsk
References
1. Zhang H., Li P., Wu D., Xu D., Hou Y., Wang Q., Li M., Li Y., Zhang F., Shi Q., Serum IgG subclasses in autoimmune diseases. Medicine, 2015, vol. 1, no. 2, pp. 387e-393. https://doi.org/10.1097/MD.0000000000000387
2. Bardakhivskaya K. L., Nikolaev V. G., Uvarov V. Yu., Tsimbalyuk R. S., Ivanyuk A. A. Immunoadsorption in the treatment of dialated cardiomyopathy. Efferentnaya i fiziko hymicheskaya meditsina = Efferent and physico-chemical medicine, 2012, no. 3, pp. 7-10 (in Russian).
3. Bosch T. Therapeutic Apheresis-State of the Art in the Year 2005. Therapeutic Apheresis and Dialysis, 2005, vol. 9, no. 6, pp. 459-468. https://doi.org/10.1111/j.1744-9987.2005.00306.x
4. Bambauer R., Latza R., Burgard D., Schiel R. Auto-immune disorders treated with therapeutic apheresis. Therapeutic Apheresis and Dialysis, 2017, vol. 21, no. 1, pp. 111-131. https://doi.org/10.1111/1744-9987.12499
5. Abdoue N. J., Lindsley H. B., Pollock A., Stechschulte D. J., Wood G. Plasmapheresis in active systemic lupus erythematosus: effects on clinical, serum and cellular abnormalities. Case report. Clinical Immunology and Immunopathology, 1981, vol. 19, no. 1, pp. 44-54. https://doi.org/10.1016/0090-1229(81)90046-5
6. Naik A. D., Menegatti S., Gurgel P. V., Carbonell R. G. Performance of hexamer peptide ligands for affinity purification of immunoglogulin G from commercial cell culture media. Journal of Chromatography A, 2011, vol. 1218, iss. 13, pp. 1691-1700. https://doi.org/10.1016/j.chroma.2010.11.071
7. Ozturk N., Tabak A., Akgol S., Denizli A. Newly synthesized bentonite-histidine (Bent-His) micro-composite affinity sorbents for IgG adsorption. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2007, vol. 301, iss 1, pp. 490-497. https://doi.org/10.1016/j.colsurfa.2007.01.026
8. Kurimotoa R., Namekawab K., Ellisd A. V., Naitoa M., Ebaraa M. Selective adsorption of globulin on nanofiber meshes for immunoadsorption therapy. New Journal of Chemistry , 2018, vol. 42, no. 4, pp. 2916-2922. https://doi.org/10.1039/c7nj04672c
9. Pustyulga Y. S., Gribovskaya O. V., Yermola E. M., Golubovich V. P. Molecular docking of ligands promising for IgG sorption from biological fluids. Vestsi Natsyyanal’nai akademii navuk Belarusi. Seryya khimichnykh navuk = Proceedings of the National Academy of Sciences of Belarus. Chemical series, 2020, vol. 56, no. 1, pp. 88-95 (in Russian). https://doi.org/10.29235/1561-8331-2020-56-1-88-95
10. Pustyulga Y. S., Gribovskaya O. V., Ermola E. M., Golubovich V. P. Selectivity of affinity sorbents based on aromatic peptides for the binding of class G immunoglobulins. Vestsi Natsyyanal’nai akademii navuk Belarusi. Seryya khimichnykh navuk = Proceedings of the National Academy of Sciences of Belarus. Chemical series, 2020, vol. 56, no. 3, pp. 333-338 (in Russian). https://doi.org/10.29235/1561-8331-2020-56-3-333-338
11. Pustyulga Y. S., Ermola E. M. Search for ligands of sorbents for the extraction of IgG from biological fluids. 2-i mezhdunar. biokhim. kongress. Sovremennye problemy biokhimii i molekulyarnoi biologii [2nd International Biochemical Congress. Modern problems of biochemistry and molecular biology]. Grodno, Institute of Biochemistry of biologically active compounds of the NAS of Belarus, 2018, pp. 482-487 (in Russian).
12. Osipov A. P., Dzantiev B. B., Gavrilova E. M. Theory and practice of enzyme immunoassay. Moscow, Vysshaya shkola Publ., 1991. 288 p. (in Russian).
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