Development of a multiplex system for determining 11 genetic markers of predisposition to obesity
https://doi.org/10.29235/1561-8331-2021-57-3-300-309
Abstract
A system has been developed to identify 11 genetic markers associated with the risk of obesity: rs10852521, rs11075990, rs1121980, rs1421085, rs1477196, rs17817449, rs3751812, rs7206790, rs8047395, rs9940128 (FTO gene) and rs1137101 (LEPR gene) by minisequencing (SNaPshot analysis). The conditions for carrying out the amplification and minisequencing reactions, as well as the compositions of the reaction mixtures, were optimized so that the analysis was carried out for all 11 markers simultaneously. The resulting system was tested and showed a high degree of reproducibility and sensitivity required for the detection of these polymorphisms.
Supporting agencies: Funding of the work was carried out within the framework of Events 6 and 9 of the Scientific and Technical Programm of the Union State “DNA identification”.
About the Authors
G. V. Sergeev
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Gennady V. Sergeev – Ph. D. (Chemistry), Head of the Laboratory
5/2, Acad. Kuprevich Str., Minsk
I. V. Haidukevich
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Irina V. Haidukevich – Ph. D. (Chemistry), Senior Researcher
5/2, Acad. Kuprevich Str., Minsk
A. M. Gorkavaya
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Anna M. Gorkavaya – Researcher
5/2, Acad. Kuprevich Str., Minsk
M. D. Ameliyanovich
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Maxim D. Ameliyanovich – Researcher
27, Akademicheskaya str., Minsk
I. B. Mosse
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Irma B. Mosse – D. S. (Biology), Professor, Principal Researcher
27, Akademicheskaya str., Minsk
S. A. Usanov
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Sergei A. Usanov – Corresponding Member of the National Academy of Sciences of Belarus, D. S. (Chemistry), Professor, Principal Researcher
5/2, Acad. Kuprevich Str., Minsk
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