Development and validation of the method for quantitative determination of preservatives in sodium oxybutyrate syrup
https://doi.org/10.29235/1561-8331-2023-59-1-26-34
Abstract
A simple, fast, sensitive method for the determination of preservatives (methyl parahydroxybenzoate, propyl parahydroxybenzoate) in the finished dosage form of sodium oxybutyrate syrup based on the simultaneous determination of preservatives using high performance liquid chromatography (HPLC) is proposed. The HPLC method was validated. The correlation coefficient of the linearity of the technique was 0.999 in the application range of 80–120 % of the normalized value. The calibration graph is linear in the region of 0.495–0.990 mg/ml of methyl 4-hydroxybenzoate and 0.165–0.330 mg/ml of propyl 4-hydroxybenzoate. It has been established that the analytical characteristics of the test procedure (selectivity, linearity, correctness, repeatability, reproducibility) satisfy the selected acceptance criteria.
About the Authors
Z. I. Kuvaeva
Institute of Physical Organic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Kuvaeva Zoya I. – D. Sc. (Chemistry), Professor, Head
of the Department
13, Surganov Str., 220072, Minsk
S. P. Kacherskaya
Institute of Physical Organic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Kacherskaya Svetlana P. – Head of the thematic group
for the analytical control of medicinal substances
13, Surganov Str., 220072, Minsk
O. S. Mastitskaya
Institute of Physical Organic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Mastitskaya Olga S. – Junior Researcher
13, Surganov Str., 220072, Minsk
E. A. Khvalova
Institute of Physical Organic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Khvalova Evgeniya A. – Junior Researcher
13, Surganov Str., 220072, Minsk
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