Synthesis of thiol-containing DNA-oligonucleotides using a phosphoramidite reagent based on trans -4-hydroxy-L-prolinol
https://doi.org/10.29235/1561-8331-2024-60-1-36-44
Abstract
In this work, a new phosphoramidite reagent for the preparation of thiol-modified oligonucleotides was synthesized. Thiol-specific reagents have been used to demonstrate the reactivity of thiol groups. After modification, conjugates were purified via gel-filtration and characterized with HPLC-MS.
Keywords
automated oligonucleotide synthesis,
phosphoramidite method,
thiol phosphoramidite,
thiol-containing oligonucleotides,
post-synthetic modification,
thiol-specific reagents
Supporting agencies: The work was supported by BRFFR-RFFR (agreement № Х21РМ-107).
About the Authors
Y. P. Lamekina
Institute of Physical Organic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Yuliya P. Lamekina – Researcher.
13, Surganov Str., 220072, Minsk
T. P. Seviarynchyk
Institute of Physical Organic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Tatsiana P. Seviarynchyk – Researcher.
13, Surganov Str., 220072, Minsk
E. A. Ulashchik
Institute of Physical Organic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Egor A. Ulashchik – Researcher.
13, Surganov Str., 220072, Minsk
T. P. Akhlamionok
Institute of Physical Organic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Tatsiana P. Akhlamionok – Junior Researcher.
13, Surganov Str., 220072, Minsk
P. Y. Baryshchyk
Institute of Physical Organic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Palina Y. Baryshchyk – Junior Researcher.
13, Surganov Str., 220072, Minsk
B. V. Ranishenka
Institute of Physical Organic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Bahdan V. Ranishenka – Ph. D. (Chemistry), Researcher.
13, Surganov Str., 220072, Minsk
V. V. Shmanai
Institute of Physical Organic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Vadim V. Shmanai – Ph. D. (Chemistry), Head of the Laboratory.
13, Surganov Str., 220072, Minsk
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