DEVELOPMENT AND VALIDATION OF THE METHOD FOR CO-DETERMINATION OF PESTICIDE RESIDUES IN WINTER WHEAT GRAIN BY GAS CHROMATOGRAPHY WITH MASS SPECTROMETRIC DETECTION

Method for determination of 69 pesticides from different classes (amides, anilinopyrimidines, dinitroanilines, imidazoles, carbamates, carbanilates, morpholines, neonicotinoids, organodithiophosphates, organothiophosphates, pyrethroids, simmtriazines, esters, strobilurins, thiocarbamates, triazoles, etc.) in winter wheat grain by gas chromatography with mass spectrometric detection has been developed and validated by experimental data. The developed extraction method of sample preparation is simple, quick, consumes only cheap and rather common reagents and provides extracts clean enough to obtain reproducible quantitative results without cleaning of injector liner at least for 200 injections. The recovery values of studied pesticides from winter wheat grain were between 76 and 118 % with RSD values below 9 % at 10.0, 20.0 and 200 µg·kg^–1 spiking levels. Detection limits were less or equal to 0.01 mg·kg⁻¹ for all the studied pesticides. The obtained limits of quantification were below or equal to the maximum residue levels (MRLs) which are set by Belarus and the European Union for the corresponding pesticides in cereal grains. The linear range used in the calibration curves was from 50 to 3000 µg·L^–1 with the values of the determination coefficients R² more than 0.997. The developed method was successfully used for the analysis of cereal grains for the residues of the pesticides, which were under registration trials held in Belarus in 2015. 

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