ФАГОВЫЙ ДИСПЛЕЙ В КОНСТРУИРОВАНИИ АНТИТЕЛ С ЗАДАННЫМИ СВОЙСТВАМИ
Аннотация
Уникальная особенность моноклональных антител высокоспецифично взаимодействовать с молекулярными мишенями позволила им занять ведущее положение в терапии онкологических и аутоиммунных заболеваний, стать незаменимым инструментом протеомных исследований и компонентом диагностических систем. Представлен обзор новейших литературных данных по использованию метода фагового дисплея для получения рекомбинантных антител, которые другими методами получить невозможно, а также собственные оригинальные результаты в этой области. На основании бионформационного анализа структур депонированных комплексов антител с антигенами нами создана комбинаторная библиотеки Fab фрагментов антител человека, обладающая разнообразием более 1010 независимых клонов и способная служить источником рекомбинантных антител. С использованием негативной селекции нами получены и характеризованы высокоспецифичные однодоменные антитела к альдостерон-синтазе (цитохром Р45011В2, CYP11B2), не обладающие в ИФА кросс-реактивностью с ее гомологом CYP11B1 (93% идентичности последовательностей), а также антитела к таким высокомолекулярным мишеням, как эритропоэтин, соматотропный гормон и тиреопероксидаза человека.
Об авторах
Д. О. Дормешкин
Институт биоорганической химии НАН Беларуси
Беларусь
науч. сотрудник
Беларусь
науч. сотрудник
Е. А. Бричко
Белорусский государственный университет
Беларусь
студент
Беларусь
студент
А. А. Гилеп
Институт биоорганической химии НАН Беларуси
Беларусь
канд. хим. наук, зав. отделом молекулярных биотехнологий
Беларусь
канд. хим. наук, зав. отделом молекулярных биотехнологий
С. А. Усанов
Институт биоорганической химии НАН Беларуси
Беларусь
член-кор., д-р хим. наук
Беларусь
член-кор., д-р хим. наук
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