COMPARATIVE ANALYSIS OF M. TUBERCULOSIS CYP124 REDUCTION WITH DIFFERENT REDOX PARTNERS
https://doi.org/10.29235/1561-8331-2018-54-2-190-196
Abstract
In order to determine the optimal redox partners of CYP124 M. tuberculosis in reconstituted in vitro system we carried out heterologous expression, isolation and purification of recombinant CYP124 Mycobacterium tuberculosis and candidate redox partners: Fdx, FdR, FprA, Adx, AdR, CPR, Arh1_A18G, Etpfd. CYP124 was shown to exhibit the highest catalytic activity in system with S. pombe mitochondrial electron transfer proteins: Etp1fd и Arh1_A18G.
About the Authors
A. M. Tumilovich
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus.
Belarus
Belarus
Anastasiya M. Tumilovich – Researcher.
5/2, Acad. V. F. Kuprevich Str., 220141, Minsk.
A. A. Kavaleuski
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus.
Belarus
Belarus
Anton A. Kavaleuski – Junior researcher.
5/2, Acad. V. F. Kuprevich Str., 220141, Minsk.
T. S. Varaksa
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus.
Belarus
Belarus
Tatsiana S. Varaksa – Junior researcher.
5/2, Acad. V. F. Kuprevich Str., 220141, Minsk.
A. V. Vasilevskaya
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus.
Belarus
Belarus
Anna V. Vasilevskaya – Ph. D. (Chemistry), Senior researcher.
5/2, Acad. V. F. Kuprevich Str., 220141, Minsk.
T. S. Cherkesova
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus.
Belarus
Belarus
Tatsiana S. Cherkesova – Researcher.
5/2, Acad. V. F. Kuprevich Str., 220141, Minsk.
I. P. Grabovets
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus.
Belarus
Belarus
Irina P. Grabovets – Researcher.
5/2, Acad. V. F. Kuprevich Str., 220141, Minsk.
T. V. Shkel
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus.
Belarus
Belarus
Tatsiana V. Shkel – Researcher.
5/2, Acad. V. F. Kuprevich Str., 220141, Minsk.
Ya. V. Dichenko
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus.
Belarus
Belarus
Yaroslav V. Dichenko – Senior researcer.
5/2, Acad. V. F. Kuprevich Str., 220141, Minsk.
A. A. Gilep
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus.
Belarus
Belarus
Andrei A. Gilep – Ph. D. (Chemistry), Head of the Laboratory.
5/2, Acad. V. F. Kuprevich Str., 220141, Minsk.
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