ENZYME IMMUNOASSAY KIT FOR THE DETERMINATION OF DEOXYNIVALENOL
https://doi.org/10.29235/1561-8331-2018-54-3-319-328
Abstract
A reagent kit EIA-DEOXYNIVALENOL for the determination of mycotoxin deoxynivalenol (DON) in feeds and foods by a direct competitive enzyme immunoassay using microtitration plate has been developed and tested. The basic components of the kit are polyclonal antibodies to DON, obtained as a result of immunization of rabbits with a conjugate of DON with bovine serum albumin and a conjugate of horseradish peroxidase with DON. The evaluated parameters of the kit and metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development and provide the determination of DON content of agricultural products in a range of 0.2 to 6.0 mg/kg with proper accuracy and precision. The limit of quantitative determination of DON in grain and cereal foods does not exceed 0.2 mg/kg.
About the Authors
I. I. Vashkevich
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Irina I. Vashkevich – Ph. D. (Chemistry), Leading Researcher
5/2, Academician V.F. Kuprevich Str., 220141, Minsk
O. S. Kuprienko
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Olga S. Kuprienko – Ph. D. (Chemistry), Senior Researcher
5/2, Academician V.F. Kuprevich Str., 220141, Minsk
I. V. Gorbachova
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Irina V. Gorbachova – Junior Researcher
5/2, Academician V.F. Kuprevich Str., 220141, Mins
A. A. Yastrebova
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Anna A. Yastrebova – Researcher
5/2, Academician V.F. Kuprevich Str., 220141, Minsk
T. V. Terentieva
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Tatiana V. Terentieva – Researcher
5/2, Academician V.F. Kuprevich Str., 220141, Minsk
G. S. Kornilovich
Central Research Laboratory of Grain Products
Belarus
Belarus
Galina S. Kornilovich – Deputy Director for Science
Minsk reg., Smolevichi distr., 222220, Oktyabrsky vil.
L. N. Sukhenko
Central Research Laboratory of Grain Products
Belarus
Belarus
Liliya N. Sukhenko – Head of Department
Minsk reg., Smolevichi distr., 222220, Oktyabrsky vil.
A. I. Shibeko
Central Research Laboratory of Grain Products
Belarus
Belarus
Anna I. Shibeko – Leading Engineer-Chemist
Minsk reg., Smolevichi distr., 222220, Oktyabrsky vil.
O. V. Sviridov
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Oleg V. Sviridov – D. Sc. (Chemistry), Head of Laboratory
5/2, Academician V.F. Kuprevich Str., 220141, Minsk
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