Direct competitive enzyme immunoassay method for the determination of ochratoxin a in feeds and foods
https://doi.org/10.29235/1561-8331-2019-55-1-69-78
Abstract
A reagent kit EIA-OCHRATOXIN A for the determination of mycotoxin ochratoxin A in feeds and foods by a direct competitive enzyme immunoassay using microtitration plate has been developed and tested. The conjugate of ochratoxin A with horseradish peroxidase was synthesized using the mycotoxin aminoderivative joined to the carbohydrate chain of the enzyme. A monoclonal antibody to the mycotoxin was immobilized on the surface of plate wells through sheep anti-mouse antibodies. The evaluated parameters of the kit and metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development and provide the determination of ochratoxin A content of agricultural products in a range of 5 to 375 mg/kg with proper accuracy and precision. The limit of quantitative determination of ochratoxin A in grain and cereal foods does not exceed 5 mg/kg.
About the Authors
I. I. Vashkevich
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Irina I. Vashkevich – Ph. D. (Chemistry), Leading Researcher.
5/2, Academician V. F. Kuprevich Str., 220141, Minsk.
O. S. Kuprienko
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Olga S. Kuprienko – Ph. D. (Chemistry), Senior Researcher.
5/2, Academician V. F. Kuprevich Str., 220141, Minsk.
I. V. Gorbachova
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Irina V. Gorbachova – Junior Researcher.
5/2, Academician V. F. Kuprevich Str., 220141, Minsk.
D. A. Semenov
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Dmitriy A. Semenov – Researcher, Institute of Bioorganic Chemistry.
5/2, Academician V. F. Kuprevich Str., 220141, Minsk.
N. P. Perebora
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Nadezhda P. Perebora – Junior Researcher.
5/2, Academician V. F. Kuprevich Str., 220141, Minsk.
A. A. Yastrebova
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Anna A. Yastrebova – Researcher, Institute of Bioorganic Chemistry.
5/2, Academician V. F. Kuprevich Str., 220141, Minsk.
G. S. Kornilovich
Central Research Laboratory of Grain Products
Belarus
Belarus
Galina S. Kornilovich – Deputy Director for Science.
Minsk reg., Smolevichi distr., 222220, Oktyabrsky vil.
L. N. Sukhenko
Central Research Laboratory of Grain Products
Belarus
Belarus
Liliya N. Sukhenko – Head of the Department.
Minsk reg., Smolevichi distr., 222220, Oktyabrsky vil.
A. I. Shibeko
Central Research Laboratory of Grain Products
Belarus
Belarus
Anna I. Shibeko – Leading Engineer-Chemist.
Minsk reg., Smolevichi distr., 222220, Oktyabrsky vil.
O. V. Sviridov
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Oleg V. Sviridov – D. Sc. (Chemistr y), Head of the Laboratory.
5/2, Academician V. F. Kuprevich Str., 220141, Minsk.
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