Direct competitive enzyme immunoassay method for the determination of ochratoxin a in feeds and foods

I. I. Vashkevich; O. S. Kuprienko; I. V. Gorbachova; D. A. Semenov; N. P. Perebora; A. A. Yastrebova; G. S. Kornilovich; L. N. Sukhenko; A. I. Shibeko; O. V. Sviridov

doi:10.29235/1561-8331-2019-55-1-69-78

Direct competitive enzyme immunoassay method for the determination of ochratoxin a in feeds and foods

I. I. Vashkevich, O. S. Kuprienko, I. V. Gorbachova, D. A. Semenov, N. P. Perebora, A. A. Yastrebova, G. S. Kornilovich, L. N. Sukhenko, A. I. Shibeko, O. V. Sviridov

https://doi.org/10.29235/1561-8331-2019-55-1-69-78

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Abstract

A reagent kit EIA-OCHRATOXIN A for the determination of mycotoxin ochratoxin A in feeds and foods by a direct competitive enzyme immunoassay using microtitration plate has been developed and tested. The conjugate of ochratoxin A with horseradish peroxidase was synthesized using the mycotoxin aminoderivative joined to the carbohydrate chain of the enzyme. A monoclonal antibody to the mycotoxin was immobilized on the surface of plate wells through sheep anti-mouse antibodies. The evaluated parameters of the kit and metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development and provide the determination of ochratoxin A content of agricultural products in a range of 5 to 375 mg/kg with proper accuracy and precision. The limit of quantitative determination of ochratoxin A in grain and cereal foods does not exceed 5 mg/kg.

Keywords

mycotoxins, ochratoxin A, enzyme immunoassay

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Proceedings of the National Academy of Sciences of Belarus, Chemical Series

Direct competitive enzyme immunoassay method for the determination of ochratoxin a in feeds and foods

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Proceedings of the National Academy of Sciences of Belarus, Chemical Series

Direct competitive enzyme immunoassay method for the determination of ochratoxin a in feeds and foods

Full Text:

Abstract

Keywords

About the Authors

References

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