Determination of the bifenazate insecticide in apples and cucumbers by high performance liquid chromatography
https://doi.org/10.29235/1561-8331-2019-55-4-422-428
Abstract
Based on the distribution constants of biphenazate, obtained experimentally and also calculated from literature data on the solubility of biphenazate in water and organic solvents, as well as experimental data on the extraction of biphenazate from plant matrices by various extractants, we selected the optimal conditions for extracting biphenazate from apples and cucumbers. The conditions for the purification of the extracts were also selected. Acetonitrile in the presence of ammonium sulfate and hexane was used for extraction of the pesticide. Purification of extracts of plant materials was carried out by partitioning between hexane and water-acetonitrile mixture. The samples obtained after this treatment were pure enough to determine the residual amounts of biphenazate in them at the maximum residue level determined in Belarus and the countries of the European Union, or lower using widespread liquid chromatography with diode-array (ultraviolet) detection.
About the Authors
M. F. Zayats
Belarusian State University
Belarus
Belarus
Mikhail F. Zayats – Ph. D. (Chemistry), Head of the Department
14, Leningradskaya Str., 220030, Minsk
S. M. Leschev
Belarusian State University
Belarus
Belarus
Sergey M. Leschev – D. Sc. (Chemistry), Professor
14, Leningradskaya Str., 220030, Minsk
References
1. Bifenazate. IUPAC. Gglobal availability of information on agrochemicals. Available at: https://sitem.herts.ac.uk/aeru/iupac/Reports/76.htm(accessed12June2019).
2. Bifenazate (219). Available at: http://www.fao.org/fileadmin/templates/agphome/documents/Pests_Pesticides/JMPR/Evaluation06/Bifenazate06.pdf (accessed 12 June 2019).
3. EURL-SRM – Analytical Method Report. Available at: http://www.eurl-pesticides.eu/userfiles/file/EurlSRM/meth_Bifenazate_EurlSRM.pdf (accessed 12 June 2019).
4. Determination of 35 Pesticides and 3 Cannabinoids in Marijuana Edibles S.O.F.T. Lunch and Learn. Available at: https://thecannabisscientist.com/fileadmin/tas/10215-app-note-UCT-supplied.pdf (accessed 12 June 2019).
5. Hengel M. J., Miller M. Analysis of pesticides in dried hops by liquid chromatography-tandem mass spectrometry. Journal of Agricultural and Food Chemistry, 2008, vol. 56, no. 16, pp. 6851–6856. https://doi.org/10.1021/jf8009624
6. Mariappan M., Kumar H., Kandaswamy C., Kulkurni S. Determination of Multi-Pesticide Residues in Red Chili Powder Using QuEChERS and the Agilent 7000 Series Triple Quadrupole GC/MS System: Application Note. Available at: http://hpst.cz/sites/default/files/uploaded_files/5991-4193en_determination_of_multipesticide_residue_in_red_chili_powder_using_quechers_and_the_agilent_7000series_qqq_gcms_system.pdf (accessed 12 June 2019).
7. MG 4.1.3100-13. Determination of residues of biphenazate and D 3598 in water, soil, apples and apple juice by high performance liquid chromatography. Methodical guidelines: approved by Rospotrebnadzor on 08.08.2013. Moscow, Federal Center of Hygiene and Epidemiology of Rospotrebnadzor, 2014. 28 p. (In Russian).
8. New pesticide fact sheet. Bifenazate. Available at: https://www3.epa.gov/pesticides/chem_search/reg_actions/registration/fs_PC-000586_01-Jun-99.pdf (accessed 12 june 2019).
9. Rudakov O. B., Selemenev V. F., Rudakova L. V., Podolina E. A. Chromatographic and extraction properties of acetonitrile and mixtures of acetonitrile with water. Sorbtsionnye i Khromatograficheskie Protsessy = Sorption and chromatographic processes, 2018, vol. 18, no. 4, pp. 458–478 (in Russian).
10. Zayats M. F., Leschev S. M. The development of methods for the determination of residues of the herbicide halauxifenmethyl in barley and rape. Zhurnal Belorusskogo gosudarstvennogo universiteta. Khimiya = Journal of the Belarusian State University. Chemistry, 2019, no. 1, pp. 59–65 (In Russian).
11. SANTE/11813/2017. Guidance document on analytical quality control and method validation procedures for pesticide residues and analysis in food and feed. Available at: https://ec.europa.eu/food/sites/food/files/plant/docs/pesticides_mrl_guidelines_wrkdoc_2017-11813.pdf (accessed 08 June 2019).
12. Resolution of the Ministry of Health of the Republic of Belarus No. 149 of 27.09.2012 “On the Approval of Sanitary Norms and Regulations” Requirements for the Application, Conditions of Transport and Storage of Pesticides (Plant Protection Products), Agrochemicals and Mineral Fertilizers”, Hygienic Standard “Hygienic Norms for the Content of Active Substances of Pesticides ( Plant protection products) in environmental objects, food raw materials, foodstuffs” and the recognition of some Resolutions invalid of the Chief Sanitary Doctor the Republic of Belarus and their individual structural elements”. Available at: https://registr.by/doc/1215904 (accessed 08 June 2019) (in Russian).
13. Plants. EU Pesticides database. Current MRL values. Available at: http://ec.europa.eu/food/plant/pesticides/eupesticides-database/public/?event=pesticide.residue.CurrentMRL&language=EN (accessed 12 June 2019).
Review
Views: 601
Email this article 