Phosphoramidite reagent and solid-phase support for synthesis of 5'- and 3'-phosphorylated oligonucleotides
Abstract
Syntheses of a phosphoramidite reagent for 5'-phosphorylation of oligonucleotides, and of a modified solid-phase support (controlled pore glass, CPG) for 3'-phosphate modification of oligonucleotides are described. Efficasy of the reagents has been confirmed by the synthesis of phosphorylated oligonucleotides. The oligonucleotides have been analyzed by electrophoresis and HPLC.
About the Authors
M. Yu. Tatulchenkov
Институт физико-органической химии НАН Беларуси
Belarus
A. R. Nabiullin
Институт физико-органической химии НАН Беларуси
Belarus
M. V. Kvach
Институт физико-органической химии НАН Беларуси
Belarus
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