Evaluation of the expression and catalytic activity of cytochrome P450 isoenzymes in cancer cell line A549
Abstract
The contribution of monooxygenase component in carcinogenic activation of a key procarcinogenic derivative of benzo(a) pyrene - 7,8-benzo(a)pyrenediol in lung adenocarcinoma cell line A549 has been evaluated. It has been shown that the contribution of monooxygenase process under the experimental conditions in A549 cells is only 13 %, which corresponds with the relatively low level of constitutive expression of CYP1A1 and CYP1B1. When cells are exposed to 20-methylcholanthrene, monooxygenase component in carcinogenic activation of 7,8-benzo(a)pyrenediol has reached 25 %. Moreover, 90 % is accounted for a "complete" carcinogen - diolepoxide-2. As 20-methylcholanthrene is a polycyclic aromatic compound and exists with a benzo(a) pyrene in the tobacco smoke, this suggests a whole pool of polycyclic aromatic hydrocarbons entering the organism may significantly affect not only the level but also the direction of carcinogenic PAH activation of individual compounds.
About the Authors
A. G. Sysa
Институт биоорганической химии НАН Беларуси
Belarus
Belarus
O. V. Panibrat
Институт биоорганической химии НАН Беларуси
Belarus
Belarus
A. S. Babenko
РНПЦ онкологии и медицинской радиологии им. Н. Н. Александрова МЗ Беларуси
Belarus
Belarus
P. S. Shabunya
Институт биоорганической химии НАН Беларуси
Belarus
Belarus
S. A. Fatykhova
Институт биоорганической химии НАН Беларуси
Belarus
Belarus
P. A. Kiselev
Институт биоорганической химии НАН Беларуси
Belarus
Belarus
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