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Proceedings of the National Academy of Sciences of Belarus, Chemical Series

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Selectivity of affinity sorbents based on aromatic peptides for the binding of class G immunoglobulins

https://doi.org/10.29235/1561-8331-2020-56-3-333-338

Abstract

Biospecific sorbents for the removal of IgG and subclasses from biological fluids based on oligopeptides that contain aromatic protein residues have been created. A functional assessment of high-quality experimental samples of sorbents and their preferences for IgG subclasses was carried out. It was found that each sorbent has good characteristics for removing IgG from biological fluids, but the sorbent based on Phe-Trp-DTyr is more effective in binding of total IgG. With respect to IgG subclasses, the best binding results are as follows: Phe-Gln-Tyr-OMe - IgG1 (86,53%), Phe-Ala-Tyr - IgG2 (60,2%), Phe-Trp-DTyr - IgG3 (59,52%) and IgG4 (55,05%).

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ISSN 1561-8331 (Print)
ISSN 2524-2342 (Online)