Validation of quantitative determination of original substance of dipeptide threonylthreonine
https://doi.org/10.29235/1561-8331-2021-57-2-152-161
Abstract
Validation of the quantitative determination of the threonylthreonine substance was carried out in terms of specificity, linearity, correctness, precision, including repeatability and reproducibility. The linearity correlation coefficient of the method was 0.99998 in the range of 80–120 % of the standardized value. The calculated Student’s coefficients for the range 80, 100, 120 % are respectively equal to 1.32; 0.31; 0.55 and do not exceed the permissible limit values. The open rate was 100.07 %. The repeatability limit for one measurement group was – 0.20; for two – 0.366 in accordance with the eligibility criteria. The calculated Student’s (t = 1.34) and Fisher’s (F = 2.00) criteria for two groups of measurements did not exceed the permissible values. The developed technique for potentiometric titration of the threonylthreonine substance in a non-aqueous medium is valid.
About the Authors
L. A. VodopyanovaBelarus
Liudmila A. Vodopyanova – Researcher
13, Surganov Str., 220072, Minsk
Z. I. Kuvaeva
Belarus
Zoya I. Kuvaeva – D. Sc. (Chemistry), Professor. Head of the Department
13, Surganov Str., 220072, Minsk
E. G. Karankevich
Belarus
Elena G. Karankevich – Ph. D. (Chemistry), Head of the Laboratory
13, Surganov Str., 220072, Minsk
E. B. Korzyuk
Belarus
Ekaterina B. Korzyuk – Junior Researcher
13, Surganov Str., 220072
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