Extraction of medicinal compounds of the benzodiazepines class from aqueous solutions and their following gas chromatographic determination
https://doi.org/10.29235/1561-8331-2021-57-2-177-184
Abstract
In extraction systems hexane–water, hexane–aqueous solutions of inorganic salts (sodium chloride, dipotassium phosphate and potassium carbonate), chloroform–water using the method of gas chromatography–mass spectrometry, the distribution constants of psychotropic substances, limited to movement through the customs border of the Republic of Belarus – alprazolam, lorazepam, temazepam, and as well as clozapine, a medicinal product permitted for movement, were determined. The greatest dynamics of the growth of the distribution constant versus salt concentration was observed in alprazolam. It was found that replacing hexane with chloroform leads to increasing the distribution constants and the quantitative extraction of all substances. However, for the selective extraction of substances from various objects and the simultaneous removal of matrix components, it is necessary to use the systems as hexane–aqueous solutions of salts or hexane–water. Based on the obtained values of the distribution constants of substances, extraction-chromatographic technique for the determination of organic nonelectrolytes (benzodiazepines) in various objects is proposed. Metrological characteristics of the techniques are: relative standard deviation does not exceed 2 %, the range of determined concentrations in solutions is 0,05– 1,00 g/dm3.
About the Authors
O. N. Mikhniuk
State Institute of Advanced Training and Retraining of Customs Authorities of the Republic of Belarus
Belarus
Belarus
Olga N. Mikhniuk – Chief expert of the Customs laboratory; Postgraduate student. Belarusian State University
45/4, Mogilevskaya Str., 220007, Minsk
14, Leningradskaya Str., 220050, Minsk
S. M. Leshchev
Belarusian State University
Belarus
Belarus
Sergei M. Leschev – D. Sc. (Chemistry), Professor
14, Leningradskaya Str., 220050, Minsk
References
1. Recommended methods for the identification and analysis of barbiturates and benzodiazepines under international control: manual for use by national drug analysis laboratories. New York: UN, 2012. Available at: https://www.unodc.org/documents/scientific/barbiturates_and_benzodiazepines.pdf
2. Recommended methods for testing lysergide (LSD): manual for use by national narcotics laboratories. New York: UN, 1989. Available at: https://www.unodc.org/pdf/publications/report_testinglsd_1989-01-01_1.pdf
3. Recommended methods for testing peyote cactus (mescalin buttons)/mescaline and psilocybe mushrooms/psilocybin: manual for use by national narcotics laboratories. New York: UN, 1989. Available at: https://www.unodc.org/pdf/publications/st-nar-19.pdf
4. Korenman I. M. Extraction in the analysis of organic substances. Moscow, Khimiya Publ., 1997. 200 p. (in Russian).
5. Leshchev S. M., Zayats M. F. Distribution constants of lower alcohols, acetone, and ethyl acetate in n-hexane – aqueous solutions of inorganic salt systems and the nature of the salting-out effect. Russian journal of physical chemistry A, 2012, vol. 86, no 6, pp. 965–968. https://doi.org/10.1134/S0036024412060167
6. Leshchev S. M., Mikhniuk O. N., Nemkevich A. V., Furs S. F. Extraction of organic nonelectrolytes with n-hexane from aqueous solutions of hydrogen phosphate and potassium acetate. Vestsi Natsiyyanal’nai akademii navuk Belarusi. Seryya khimichnykh navuk = Proceedings of the National Academy of Sciences of Belarus. Chemical series, 2019, vol. 55, no. 2, pp. 149–155 (in Russian). https://doi.org/10.29235/1561-8331-2019-55-2-149-155
7. Leshchev S. M., Mikhniuk O. N., Kryzhny K. D., Zayats M. F. The use of salting out to extract hydrophilic physiologically active substances from aqueous solutions for their further chromatographic determination. Analitika i kontrol’ = Analytics and Control, 2019, vol. 23, no. 4, pp. 494–500 (in Russian). https://doi.org/10.15826/analitika.2019.23.4.004
8. Moffat A. C., Osselton M. D., Widdop B., Watts J. Clarke’s Analysis of Drugs and Poisons in pharmaceuticals, body fluids and postmortem material. London: Pharmaceutical Press Publ., 2011. 1048 p.
9. Mikhniuk O. N., Leshchev S. M., Kasyanchik P. A. Distribution constants and group increments of organic substances in extraction systems aqueous salt solution – hexane. Vestsi Natsiyyanal’nai akademii navuk Belarusi. Seryya khimichnykh navuk = Proceedings of the National Academy of Sciences of Belarus. Chemical series, 2020, vol. 56, no. 2, pp. 158–165 (in Russian). doi.org/10.29235/1561-8331-2020-56-2-158-165
10. Eremin S. K., Izotov B. N., Veselovskaja N. V. Analysis of narcotic drugs: a guideline to the chemical and toxicological analysis of narcotic and other intoxicating substances. Мoscow, Mysl’ Publ., 1993. 259 p. (in Russian).
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