Hydrolysis of UV-induced peroxidized phosphatidylcholine initiated by phospholipases of different substrate specificities
https://doi.org/10.29235/1561-8331-2021-57-2-195-205
Abstract
The activity of porcine pancreatic phospholipase A2 and the same of cobra venom toward phosphatidylcholine having different supramolecular organization and interfacial charge (micelles with sodium deoxycholate and liposomes) under UV irradiation (180–400 nm) was studied. It was shown that the UV-irradiated lipid phase is characterized by an increased index of phosphatidylcholine oxidation and the absence of a peak with a maximum of 235.5 nm, related to the presence of unsaturated bonds in the UV spectrum of docosahexaenoic acid, but retained in the presence of the antioxidant trolox. The activation of both phospholipases A2 after UV irradiation of the substrate was established, regardless of its supramolecular organization, the charge of the interfacial surface, and the substrate specificity of the enzymes. Using dynamic light scattering, 0.3 % of larger particles were found among the irradiated micelles of phosphatidylcholine. The results obtained indicate that areas of accumulation of hydroperoxidized lipids can be formed in the irradiated model membrane, which serve as a site of intensified attack for phospholipases.
Keywords
phospholipase A2 of porcine pancreas,
phospholipase A2 of cobra venom,
LPO,
micelles,
liposomes,
phosphatidylcholine
Supporting agencies: Author is grateful to all employees of the Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus for their help in carrying out the experiment proposed for this work.
About the Author
N. M. Litvinko
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus
Belarus
Belarus
Natalia M. Litvinko – D. Sc. (Chemistry), Associate Professor, Head of the laboratory
5/2, Kuprevich Str., 220141, Minsk
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