Optimization of the technology for obtaining guide RNA using plate automatic synthesizer
https://doi.org/10.29235/1561-8331-2022-58-4-398-406
Abstract
A synthetic protocol was developed and optimal reagents have been selected for obtaining guide RNA oligonucleotides for the CRISPR/Cas system using ASM-2000 automatic synthesizer in 500 nmol scale. Methods for the isolation, purification and analytical control of synthetic RNA oligonucleotides have been developed. The improved technology has been used for preparation of guide RNAs for the CRISPR Cas12a system.
About the Authors
E. A. Ulashchik
Shmanai Institute of Physical Organic Chemistry, National Academy of Sciences of Belarus
Russian Federation
Russian Federation
Ulashchik Egor A. – Researcher.
13, Surganov Str., 220072, Minsk
T. P. Akhlamionok
Shmanai Institute of Physical Organic Chemistry, National Academy of Sciences of Belarus
Russian Federation
Russian Federation
Akhlamionok Tatsiana P. – Junior Researcher.
13, Surganov Str., 220072, Minsk
P. Y. Baryshchyk
Shmanai Institute of Physical Organic Chemistry, National Academy of Sciences of Belarus
Russian Federation
Russian Federation
Baryshchyk Palina Y. – Junior Researcher.
13, Surganov Str., 220072, Minsk
O. L. Sharko
Shmanai Institute of Physical Organic Chemistry, National Academy of Sciences of Belarus
Russian Federation
Russian Federation
Sharko Olga L. – Ph. D. (Chemistry), Leading Researcher.
13, Surganov Str., 220072, Minsk
V. V. Shmanai
Institute of Physical Organic Chemistry, National Academy of Sciences of Belarus
Russian Federation
Russian Federation
Shmanai Vadim V. – Ph. D. (Chemistry), Head of the Laboratory.
13, Surganov Str., 220072, Minsk
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