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Proceedings of the National Academy of Sciences of Belarus, Chemical Series

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Heterologous expression, isolation, purification, physical and chemical properties of recombinant human cytochrome CYP1B1

Abstract

In order to study the catalytic properties of CYP1B1 toward a number of steroid compounds, we have developed an effective system of heterologous expression of hemoprotein in E.coli and isolated the preparation of recombinant CYP1B1 «soluble» form in a highly purified state. The interaction parameters of the purified enzyme with steroid ligands have been determined.
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ISSN 1561-8331 (Print)
ISSN 2524-2342 (Online)