Heterologous expression, isolation, purification, physical and chemical properties of recombinant human cytochrome CYP1B1
Abstract
In order to study the catalytic properties of CYP1B1 toward a number of steroid compounds, we have developed an effective system of heterologous expression of hemoprotein in E.coli and isolated the preparation of recombinant CYP1B1 «soluble» form in a highly purified state. The interaction parameters of the purified enzyme with steroid ligands have been determined.
About the Authors
A. J. Lushchyk
Институт биоорганической химии НАН Беларуси
Belarus
Belarus
A. A. Gilep
Институт биоорганической химии НАН Беларуси
Belarus
Belarus
I. P. Grabovets
Институт биоорганической химии НАН Беларуси
Belarus
Belarus
S. A. Usanov
Институт биоорганической химии НАН Беларуси
Belarus
Belarus
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