Chemical modification of protein with europium polycarboxylate complexonates
Abstract
A new technique for chemical modification of proteins with polycarboxylate metalochelates has been proposed. The conjugates of immunoglobulin and streptavidin, a bacterial protein, with Eu3+ complexonates have been synthesized. Protein metallochelate complexes prepared using diethylenetriaminepentaacetic acid dianhydride have been compared to those prepared from mono-N-hydroxysuccinimide ester of Eu3+ polycarboxylate complexonate, using dissociation-based timeresolved fluoroimmunoassay systems.
About the Authors
O. S. Garbuz
Институт биоорганической химии НАН Беларуси
Belarus
Belarus
I. I. Vashkevich
Институт биоорганической химии НАН Беларуси
Belarus
Belarus
O. V. Sviridov
Институт биоорганической химии НАН Беларуси
Belarus
Belarus
References
1. Hemmila I., Dakubu S., Mukkala V.-M. et al. // Anal. Biochem. 1984. Vol. 137, N 2. P. 335-343.
2. Mukkala V. M., Mikola H., Hemmila I. // Anal. Biochem. 1989. Vol. 176, N 2. P. 319-325.
3. Соколова М. В., Помелова В. Г., Чудинов А. В. и др. // Вопросы вирусологии. 1991. № 2. С. 140-142.
4. Гарбуз О. С. // Молодежь в науке - 2012: прил. к журн. «Весцi НАН Беларусi». Сер. хiм. навук. Ч. 1. 2012. С. 15-18.
5. Pilch P. F., Czech M. P. // J. Biol. Chem. 1979. Vol. 254, N 9. P. 3375-3381.
6. Najafi А., Childs R. L., Hnatowich D. J. // Int. J. Appl. Radiat. Isot. 1984. Vol. 35, N 6. P. 554-557.
7. Paxton R. J., Jakowatz J. G., Beatty J. D. et al. // Cancer Res. 1985. Vol. 45. P. 5694-5699.
8. Paik C. H., Ebbert M. A., Murphy P. R. et al. // J. Nucl. Med. 1983. Vol. 24, N 12. P. 1158-1163.
9. Green N. M. // Adv. Prot. Chem. 1975. Vol. 29. P. 85-133.
Review
Views: 586
Email this article 