Chemical modification of protein with europium polycarboxylate complexonates

Abstract

A new technique for chemical modification of proteins with polycarboxylate metalochelates has been proposed. The conjugates of immunoglobulin and streptavidin, a bacterial protein, with Eu³⁺ complexonates have been synthesized. Protein metallochelate complexes prepared using diethylenetriaminepentaacetic acid dianhydride have been compared to those prepared from mono-N-hydroxysuccinimide ester of Eu³⁺ polycarboxylate complexonate, using dissociation-based timeresolved fluoroimmunoassay systems.