SYNTHESIS OF DEUTERIUM LABELLED METHYL CHLOROFORMATE
Abstract
Most analytical methods in metabolomics allow determination of relative intensities or require several internal standards of metabolites. For quantitative analysis, determination of absolute concentrations is required. For this purpose, the gas chromatography/tandem mass spectrometry methods are used, based on methyl chloroformate derivatization and quantification by spiking samples with metabolite standards separately derivatized with deuterated derivatization reagents. Described herein is the synthesis of deuterated methyl chloroformate – the reagent for absolute quantitative metabolite analysis by gas chromatography-mass spectrometry. The method allows preparation and isolation of deuteromethylchloroformate with high yields.
About the Authors
S. Tumanov
Beatson Institute for Cancer Research, Institute of Physical Organic Chemistry of the National Academy of Sciences of Belarus
United Kingdom
Postdoctoral Research Assistant
United Kingdom
Postdoctoral Research Assistant
I. L. Lysenko
Institute of Physical Organic Chemistry of the National Academy of Sciences of Belarus
Belarus
Ph. D. (Chemistry), Senior Scintific Researcher
Belarus
Ph. D. (Chemistry), Senior Scintific Researcher
O. L. Sharko
Institute of Physical Organic Chemistry of the National Academy of Sciences of Belarus
Belarus
Ph. D. (Chemistry), Senior Researcher
Belarus
Ph. D. (Chemistry), Senior Researcher
Yu. Zubenko
Independent Petroleum Laboratory Ltd
New Zealand
Ph. D. (Chemistry), Research Assistant
New Zealand
Ph. D. (Chemistry), Research Assistant
V. V. Shmanai
Institute of Physical Organic Chemistry of the National Academy of Sciences of Belarus
Belarus
Ph. D. (Chemistry), Head of Laboratory of Bioconjugate Chemistry.
Belarus
Ph. D. (Chemistry), Head of Laboratory of Bioconjugate Chemistry.
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