PREPARATION OF PROTEIN CONJUGATES AND MONOCLONAL ANTIBODIES FOR IMMUNOASSAY OF TETRACYCLINE

Abstract

We report herein on the methods to introduce a desirable number of modifications into bovine serum albumin and horseradish peroxidase in a controlled manner. The low modification to protein ratio is required when the biological activity of the enzyme has to be preserved, and the high modification to protein ratio is needed when producing immunogens. By varying the linker and the site of antigen binding with the carrier protein, we were able to produce the antibodies of high specificity. Protein conjugates of tetracycline were synthesized and characterized. Immunization of mice was carried out and monoclonal antibodies for immunoassay of tetracycline were obtained and tested in ELISA, IC50=5 ug/ml.