Expression and purification of recombinant thermostable DNA-binding protein Sso7d
https://doi.org/10.29235/1561-8331-2023-59-3-225-233
Abstract
The Sso7d protein has exceptional structural stability and the ability to bind highly specifically to DNA, which makes the protein a promising module for creating fusion proteins and test systems. Sso7d is a part of fusion high-fidelity DNA polymerases capable of carrying out the polymerase chain reaction even in the presence of PCR inhibitors. Application of faster, simpler, and more efficient method for protein production will significantly reduce the cost of creating biosensors and conducting analyzes. This paper describes a new efficient method for obtaining recombinant Sso7d protein with a high degree of purity without using affinity chromatography.
Keywords
Sso7d,
heterologous expression,
DNA-binding proteins,
protein isolation,
ultrafiltration,
recombinant protein
Supporting agencies: The study was financially supported by the National Academy of Sciences of Belarus (grant no. 2022-27-009).
About the Authors
A. B. Sachanka
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Sachanka Antos B. – Junior Researcher, Postgraduate Student
5/2, Kuprevich Str., 220141, Minsk
M. Trawkina
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Trawkina Maria – Researcher
5/2, Kuprevich Str., 220141, Minsk
V. V. Shchur
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Shchur Veronika V. – Researcher
5/2, Kuprevich Str., 220141, Minsk
S. A. Usanov
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Usanov Sergei A. – Corresponding Member of the National Academy of Sciences of Belarus, D. Sc. (Chemistry), Professor
5/2, Kuprevich Str., 220141, Minsk
A. V. Yantsevich
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Yantsevich Aliaksei V. – Ph. D. (Chemistry), Associate Professor, Head of the Laboratory
5/2, Kuprevich Str., 220141, Minsk
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