DETERMINATION OF VULCANIZATION ACCELERATORS IN ANALYSIS OF POLYMER MATERIALS BY HPLC

Abstract

A method of simultaneous determination of altax, captax, thiuram D, thiuram E, thimate and ethylthimate in aqueous extracts in the sanitary-chemical analysis by high performance liquid chromatography has been developed. Determination was based on the gradient separation of altax, captax, thiuram D, thiuram E, thimate, ethylthimate extracted by water from test objects using Waters XTerra MS C18 column of 250 mm length, internal diameter 4.6 mm, graining phase 5 μm, while UV detection wavelengths were 265 nm and 320 nm. Retention times were 10.3±0.2 min for altax, 3.6±0.2 min for captax, 9.0±0.2 min for thiuram D, 12.3±0.2 min for thiuram E, 11.0±0.2 min for thimate, 15.5±0.2 min for ethylthimate. It has been shown that the method is linear in the range of 0.05–0.60 mkg/ml for altax, 0.005–0.60 mkg/ml for captax, 0.005–0.75 mkg/ml for thiuram D and thiuram Е, 0.01–0.90 mkg/ml for thimate and ethylthimate. Using the calibration graph and standard deviations of analytical signal, following limits of quantification were calculated: 0.01 mkg/ml for altax, 0.002 mkg/ml for captax, 0.003 mkg/ml for thiuram D, 0.005 mkg/ml for thiuram Е, 0.01 mkg/ml for thimate and ethylthimate.