Exclusion liquid chromatography of carboxymethylchitin and carboxymethylchitosan from crustacean Artemia Parthenogenetica of the Aral Sea
https://doi.org/10.29235/1561-8331-2024-60-1-45-52
Abstract
The article deals with the synthesis of water-soluble derivatives of chitin and chitosan from cysts of crustaceans of the genus Artemia Parthenogenetica of the Aral Sea by carboxymethylation of OH and NH2 groups of their chains. The elution, viscosity and polyelectrolyte properties in size exclusion liquid chromatography of carboxymethylchitin and carboxymethylchitosan were studied. The suppression of electrostatic effects and the implementation of the exclusion mechanism for the separation of these polysaccharides were achieved by using an aqueous solution of sodium nitrate with a concentration of 0.1 mol/l as an eluent, which was recommended for determining molecular weight characteristics.
About the Authors
A. S. Boymirzaev
Namangan Institute of Engineering and Technology
Uzbekistan
Uzbekistan
Azamat S. Boymirzaev – D. Sc. (Chemistry), Professor, Namangan Institute of Engineering and Technology.
7, Kasansay Str., Namangan
G. E. Berdimbetova
Karakalpak Research Institute of Natural Sciences, Karakalpak Branch of the Academy of Sciences of the Republic of Uzbekistan
Uzbekistan
Uzbekistan
Gulsara Y. Berdimbetova – Ph. D. (Chemistry), Senior Researcher, Karakalpak Scientific Research Institute of Natural Sciences.
41, Berdak Str., Nukus, Republic of Karakalpakstan
B. P. Karlybaeva
Karakalpak Research Institute of Natural Sciences, Karakalpak Branch of the Academy of Sciences of the Republic of Uzbekistan
Uzbekistan
Uzbekistan
Bakhtygul P. Karlybaeva – Doctoral Student, Karakalpak Scientific Research Institute of Natural Sciences.
41, Berdak Str., Nukus, Republic of Karakalpakstan
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